Genotypic characterization of bacteria cultured from duck faeces

• Published in: Journal of applied microbiology 2005-01, Vol.99 (2), p.301-309
• Main Authors: Murphy, J, Devane, M.L, Robson, B, Gilpin, B.J
• Format: Article
• Language: English
• Subjects: 16S rRNA; Anatidae; animal pathogenic bacteria; Animals; Bacillus; Bacillus - genetics; Bacillus - isolation & purification; Bifidobacterium - genetics; Bifidobacterium - isolation & purification; Biological and medical sciences; Campylobacter; Campylobacter - genetics; Campylobacter - isolation & purification; Clostridium; Clostridium - genetics; Clostridium - isolation & purification; Colony Count, Microbial - methods; Corynebacterium; Corynebacterium - genetics; Corynebacterium - isolation & purification; Ducks; Enterococcus; Escherichia; Escherichia - genetics; Escherichia - isolation & purification; faecal source indicators; feces; Feces - microbiology; Fundamental and applied biological sciences. Psychology; genetic variation; Genotype; Microbiology; microflora; Neisseria; Neisseria - genetics; Neisseria - isolation & purification; pathogens; Peptostreptococcus; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; sequencing; Staphylococcus; Staphylococcus - genetics; Staphylococcus - isolation & purification; Streptococcus; Streptococcus - genetics; Streptococcus - isolation & purification; Veillonella; Veillonella - genetics; Veillonella - isolation & purification
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2005.02590.x

Aims: To characterize the bacterial composition of mallard duck faeces and determine if novel bacterial species are present that could be utilized as potential indicators of avian faecal contamination. Methods and Results: Combined samples of fresh faeces from four ducks were serially diluted and plated onto six different media selected to allow the growth of a range of organisms at 42 degrees C under three atmospheric conditions: aerobic, microaerophilic and anaerobic. Forty-seven morphologically dissimilar isolates were purified and partial sequencing of the 16S rRNA indicated at least 31 bacterial species. Twenty of these could be identified to the species level including pathogenic species of Bacillus, Campylobacter, Clostridium and Streptococcus. Other species identified included: Enterococcus, Escherichia, Megamonas, Cellulosimicrobium, Neisseria, Staphylococcus and Veillonella. Potentially novel species, which could represent bacteria specific to avian fauna included Bacillus, Corynebacterium, Macrococcus and Peptostreptococcus, while four isolates had