Hibernation induction trigger reduces hypoxic damage of swine skeletal muscle

• Published in: Muscle & nerve 2005-08, Vol.32 (2), p.200-207
• Main Authors: Hong, Jinback, Sigg, Daniel C., Coles Jr, James A., Oeltgen, Peter R., Harlow, Henry J., Soule, Charles L., Iaizzo, Paul A.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Blood Proteins - metabolism; Blood Proteins - pharmacology; Electrodiagnosis. Electric activity recording; Fundamental and applied biological sciences. Psychology; Hibernation - physiology; hibernation induction trigger; Hypoxia - drug therapy; Hypoxia - physiopathology; Hypoxia - prevention & control; Investigative techniques, diagnostic techniques (general aspects); Ischemic Preconditioning - methods; isometric twitch force; Marmota - physiology; Medical sciences; Mitochondria - drug effects; Mitochondria - metabolism; Muscle Contraction - drug effects; Muscle Contraction - physiology; Muscle Fatigue - drug effects; Muscle Fatigue - physiology; muscle hypoxia; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Muscle, Skeletal - physiopathology; Narcotic Antagonists - pharmacology; Nervous system; opioid blockers; Potassium Channel Blockers - pharmacology; Potassium Channels - drug effects; Potassium Channels - metabolism; Proteins - metabolism; Proteins - pharmacology; Receptors, Opioid - drug effects; Receptors, Opioid - metabolism; Recovery of Function - drug effects; Recovery of Function - physiology; reperfusion injury; Reperfusion Injury - drug therapy; Reperfusion Injury - physiopathology; Reperfusion Injury - prevention & control; Seasons; Striated muscle. Tendons; Sus scrofa; Vertebrates: osteoarticular system, musculoskeletal system
• ISSN: 0148-639X; 1097-4598
• DOI: 10.1002/mus.20354

A link between the cardioprotective benefits of pharmacological preconditioning and natural mammalian hibernation is considered to involve the cellular activation of opioid receptors and subsequent opening of KATP channels. In previous studies, we have demonstrated the protective effects of specific δ‐opioid agonists against porcine cardiac ischemia/reperfusion injury. We hypothesize here that preincubation with hibernation induction trigger (HIT) should confer a similar protection in skeletal muscles. Therefore, muscle bundles from swine were pretreated with plasma from hibernating woodchucks (HWP) for 30 min, then exposed to hypoxia for 90 min and reoxygenation for 120 min. Stimulated twitch forces were assessed. The functional effects of pretreatment with nonhibernation (summer) woodchuck plasma, a KATP blocker, or opioid antagonist were also studied. During the reoxygenation period, significantly greater force recoveries were observed only for bundles pretreated with HWP; this response was blocked by naloxone (P < 0.05). We conclude that HIT pretreatment could be used to confer protection against hypoxia/reperfusion injury of skeletal muscles of nonhibernators; it could potentially be utilized to prevent injury during surgical procedures requiring ischemia. © 2005 Wiley Periodicals, Inc. Muscle Nerve, 2005