Evaluation of column bleed by using an ultraviolet and a charged aerosol detector coupled to a high-temperature liquid chromatographic system

In this study, five different HPLC columns were heated to 200 °C using a homemade heating system which can be operated in temperature programmed mode. The column bleed as an indicator of induced degradation of the stationary phase material was evaluated using a charged aerosol detector (CAD) and an...

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Bibliographic Details
Published in:Journal of Chromatography A 2006-06, Vol.1119 (1), p.197-201
Main Authors: Teutenberg, T., Tuerk, J., Holzhauser, M., Kiffmeyer, T.K.
Format: Article
Language:English
Subjects:
Aerosols
Analytical chemistry
Charged aerosol detection
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - standards
Exact sciences and technology
High-temperature HPLC
Hot Temperature
HPLC
Hydrogen-Ion Concentration
Online Systems
Other chromatographic methods
Stationary phase stability
Temperature programming
Ultraviolet detection
Ultraviolet Rays
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Summary:In this study, five different HPLC columns were heated to 200 °C using a homemade heating system which can be operated in temperature programmed mode. The column bleed as an indicator of induced degradation of the stationary phase material was evaluated using a charged aerosol detector (CAD) and an ultraviolet diode array detector (UV-DAD) at different wavelengths. The silica based C-18 stationary phase gave the highest bleed, and the carbon clad titanium dioxide column the lowest bleed. This was independent of both the detection technique and the wavelength.
ISSN:0021-9673
DOI:10.1016/j.chroma.2005.12.011