Platelets do not adsorb HLA class I molecules during storage of pooled platelet concentrates

• Published in: Transfusion medicine (Oxford, England) England), 2006-06, Vol.16 (3), p.176-183
• Main Authors: Zeiler, T., Heim, M., Dempfle, A., Kretschmer, V.
• Format: Article
• Language: English
• Subjects: Adsorption; Antibodies, Monoclonal; Antibody Specificity; Blood Platelets - metabolism; Blood Preservation; Flow Cytometry; Histocompatibility Antigens Class I - metabolism; HLA-A2 Antigen - metabolism; HLA-antigen; Humans; platelet; platelet storage; platelet transfusion; Platelet Transfusion - standards
• ISSN: 0958-7578; 1365-3148
• DOI: 10.1111/j.1365-3148.2006.00664.x

The origin of HLA class I molecules on platelets is still under discussion. Adsorption of HLA molecules on platelets using specific experimental conditions has been described. The study presented investigates whether there is a significant elution and adsorption of HLA class I molecules on platelets during storage of pooled random platelet concentrates (PRPC) under routine conditions. Platelet concentrates (PCs) from whole blood were prepared from HLA‐A2‐positive and HLA‐A2‐negative donors, pooled and stored under routine conditions. In addition, platelets from HLA‐A2‐negative donors were pelleted and resuspended in cell‐free plasma from HLA‐A2‐positive donors. HLA‐A2‐positive PCs (positive control), HLA‐A2‐negative PCs (negative control) and HLA‐A2‐negative platelets in plasma from HLA‐A2‐negative donors were stored simultaneously. Binding of FITC‐conjugated monoclonal murine antihuman HLA‐A2 antibodies (anti‐HLA‐A2‐mab) was measured during 5‐day storage by flow cytometry. An increased binding of anti‐HLA‐A2‐mab during storage was found on HLA‐A2‐negative platelets (P