Determination of diarylheptanoids from Alpinia officinarum (lesser galangal) by HPLC with photodiode array and electrochemical detection

Normal-phase column chromatography followed by semi-preparative reversed-phase HPLC has been used to isolate, from the rhizomes of Alpinia officinarum, five diarylheptanoids identified as 5-hydroxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone, 5-methoxy-7-(4"-hydroxy-3"-m...

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Bibliographic Details
Published in:Phytochemical analysis 2005-07, Vol.16 (4), p.252-256
Main Authors: Liu, Z, Sang, S, Hartman, T.G, Ho, C.T, Rosen, R.T
Format: Article
Language:English
Subjects:
Alpinia
Alpinia - chemistry
Alpinia officinarum
chemical analysis
Chromatography, High Pressure Liquid - methods
diarylheptanoids
Drugs, Chinese Herbal - analysis
electrochemistry
Electrochemistry - methods
heptane
Heptanes - isolation & purification
high performance liquid chromatography
HPLC-ECD
HPLC-PAD
Ketones - isolation & purification
lesser galangal
Mass Spectrometry
Molecular Structure
Nuclear Magnetic Resonance, Biomolecular
phytochemicals
plant extracts
Rhizome - chemistry
spectral analysis
Spectrophotometry, Ultraviolet
Zingiberaceae
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Summary:Normal-phase column chromatography followed by semi-preparative reversed-phase HPLC has been used to isolate, from the rhizomes of Alpinia officinarum, five diarylheptanoids identified as 5-hydroxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone, 5-methoxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone, 7-(4"-hydroxyphenyl)-1-phenylhept-4-en-3-one, 7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-hept-4-en-3-one, 1,7-diphenylhept-4-en-3-one. The levels of these five diarylheptanoids in root material were determined quantitatively by HPLC with UV detection and the assay methods so developed were simple, rapid and accurate. Four of the diarylheptanoids could also be detected by HPLC with electrochemical detection (ECD) in the oxidative mode, and ECD was found to have a higher sensitivity than photodiode array detection.
ISSN:0958-0344
1099-1565
DOI:10.1002/pca.827