Liver injury and viremia in mice infected with dengue-2 virus

The goal of this study was to test the feasibility of BALB/c mice as an experimental model in the study of dengue disease. BALB/c mice were intraperitoneal infected with DENV-2 obtained from a human patient. Histopathological analysis of infected animals revealed liver injury with viral antigens det...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2005-08, Vol.338 (2), p.236-246
Main Authors: Paes, M.V., Pinhão, A.T., Barreto, D.F., Costa, S.M., Oliveira, M.P., Nogueira, A.C., Takiya, C.M., Farias-Filho, J.C., Schatzmayr, H.G., Alves, A.M.B., Barth, O.M.
Format: Article
Language:English
Subjects:
alanine transaminase
animal disease models
Animals
Antigens, Viral - isolation & purification
aspartate transaminase
Base Sequence
Cell Line
dengue
Dengue - pathology
Dengue - virology
Dengue virus
Dengue Virus - genetics
Dengue Virus - isolation & purification
Dengue Virus - pathogenicity
Dengue virus type 2
DENV-2
Disease Models, Animal
DNA Primers
genome
Genome, Viral
Histopathology
Humans
Liver
Liver - pathology
Liver - ultrastructure
Liver - virology
liver injury
Mice
molecular sequence data
Mouse
nucleotide sequences
Reverse Transcriptase Polymerase Chain Reaction
Transaminases
Vacuoles - pathology
Vacuoles - virology
viral nonstructural proteins
viral proteins
Viremia
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Summary:The goal of this study was to test the feasibility of BALB/c mice as an experimental model in the study of dengue disease. BALB/c mice were intraperitoneal infected with DENV-2 obtained from a human patient. Histopathological analysis of infected animals revealed liver injury with viral antigens detection. In initial stages, the most prominent lesions were vacuolization and diffuse steatosis in hepatocytes. Serum levels of ALT and AST increased progressively, reaching the highest values 7 days p.i. and decreasing at the 14th day. Since levels of circulating virus were very low, viremia was analyzed in C6/36 cells. Virus presence was detected by ultrastructural analysis, confirmed by RT-PCR assays. Period of viremia was analyzed by flow cytometry with cells incubated with mouse-infected sera collected in different days, revealing peak virus levels at the 7th day p.i. All such data correlate to the development of the disease described in humans.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2005.04.042