Interleukin-6 promotes human epidermal keratinocyte proliferation and keratin cytoskeleton reorganization in culture

We have studied the effects of interleukin-6 (IL-6) on human epidermal keratinocytes by using serum-free culture conditions that allow the serial transfer, differentiation, and formation of well-organized multilayered epithelia. IL-6 at 2.5 ng/ml or higher concentrations promoted keratinocyte prolif...

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Bibliographic Details
Published in:Cell and tissue research 2006-07, Vol.325 (1), p.77-90
Main Authors: Hernández-Quintero, Miriam, Kuri-Harcuch, Walid, González Robles, Arturo, Castro-Muñozledo, Federico
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - metabolism
Cell Culture Techniques
Cell Division - drug effects
Cells, Cultured
Culture Media, Serum-Free
Dose-Response Relationship, Drug
Epidermal Growth Factor - pharmacology
Epidermis - cytology
Humans
Immunohistochemistry
Interleukin-6 - pharmacology
Keratinocytes - drug effects
Keratinocytes - physiology
Keratinocytes - ultrastructure
Keratins - metabolism
Keratins - ultrastructure
Transforming Growth Factor alpha - pharmacology
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Summary:We have studied the effects of interleukin-6 (IL-6) on human epidermal keratinocytes by using serum-free culture conditions that allow the serial transfer, differentiation, and formation of well-organized multilayered epithelia. IL-6 at 2.5 ng/ml or higher concentrations promoted keratinocyte proliferation, with an ED(50) of about 15 ng/ml and a maximum effect at 50 ng/ml. IL-6 was 10-fold less potent than epidermal growth factor (EGF) or transforming growth factor-alpha (TGF-alpha) and supported keratinocyte growth for up to eight cumulative cell generations. IL-6-treated keratinocytes formed highly stratified colonies with a narrower proliferative/migratory rim than those keratinocytes stimulated with EGF or TGF-alpha; confluent epithelial sheets treated with IL-6 also underwent an increase in the number of cell layers. We also examined the effect of IL-6 on the keratin cytoskeleton. Immunostaining with anti-K16 monoclonal antibodies showed that the keratin network was aggregated and reorganized around cell nucleus and that this was not attributable to changes in keratin levels. This is the first report concerning the induction of the reorganization of keratin intermediate filaments by IL-6 in human epidermal keratinocytes.
ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-006-0173-9