Viral load, organ distribution, histopathological lesions, and cytokine mRNA expression in goats infected with a molecular clone of the caprine arthritis encephalitis virus

Caprine arthritis encephalitis virus (CAEV) is a lentivirus of goats that causes persistent infection characterized by the appearance of inflammatory lesions in various organs. To define the sites of persistence, 5 goats were infected with a molecular clone of CAEV, and the viral load was monitored...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2006-06, Vol.350 (1), p.116-127
Main Authors: Ravazzolo, Ana Paula, Nenci, Chiara, Vogt, Hans-Rudolf, Waldvogel, Andreas, Obexer-Ruff, Gabriela, Peterhans, Ernst, Bertoni, Giuseppe
Format: Article
Language:English
Subjects:
Animal genetic
Animals
Arthritis-Encephalitis Virus, Caprine - genetics
Arthritis-Encephalitis Virus, Caprine - pathogenicity
Arthritis-Encephalitis Virus, Caprine - physiology
CAEV
Caprine arthritis encephalitis virus
caprine arthritis-encephalitis
Cloning, Molecular
Cytokine
cytokines
Cytokines - genetics
Female
Gene Expression Regulation
Goat Diseases - metabolism
Goat Diseases - pathology
Goat Diseases - virology
goats
Goats - virology
histopathology
Joints - pathology
Lentivirus
Lentivirus Infections - veterinary
Lentivirus Infections - virology
lesions (animal)
Male
messenger RNA
MVV
Primates
Real-time PCR
RNA, Messenger - genetics
RNA, Messenger - metabolism
Small ruminant lentiviruses
SRLV
Viral Load
Virus Replication
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Summary:Caprine arthritis encephalitis virus (CAEV) is a lentivirus of goats that causes persistent infection characterized by the appearance of inflammatory lesions in various organs. To define the sites of persistence, 5 goats were infected with a molecular clone of CAEV, and the viral load was monitored by real-time-PCR and RT-PCR in different sites 8 years after infection. The lymph nodes proved to be an important virus reservoir, with moderate virus replication relative to what is reported for lentiviruses of primates. Mammary gland and milk cells were preferred sites of viral replication. The viral load varied significantly between animals, which points to an important role of the genetic background. We found a clear association between occurrence of histopathological lesions and viral load in specific sites. The mRNA expression analysis of several cytokines did not reveal differences between animals that could explain the considerable individual variations in viral load observed.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2006.02.014