Effect of the 1,450 nm diode non-ablative laser on collagen expression in an artificial skin model

• Published in: Lasers in surgery and medicine 2005-07, Vol.37 (1), p.97-102
• Main Authors: Nouri, Keyvan, Zhang, Yan-Ping, Singer, Lauren, Zhu, Linjian, Huo, Ran, Ricotti, Carlos, Prodanovich, Srdjan, Li, Jie
• Format: Article
• Language: English
• Subjects: Apligraf; Collagen - biosynthesis; Collagen - metabolism; Collagen - radiation effects; collagen expression; Humans; Infant, Newborn; Low-Level Light Therapy - methods; Models, Biological; non-ablative laser; Skin, Artificial
• ISSN: 0196-8092; 1096-9101
• DOI: 10.1002/lsm.20189

Background and Objective The 1,450‐nm Smoothbeam™ Laser is a diode laser equipped with a cryogen cooling spray. Primary objectives were to evaluate the effects of this non‐ablative laser on Apligraf® (bioengineered skin‐substitute) and to document its use as a model for non‐ablative procedures. We also measured the effects of laser fluence levels on collagen and elastin expression. Study Design/Materials and Methods Three sheets of Apligraf® were used for this study. Each received six separate laser applications at 4J, 6J, 8J, 10 J, 12J, and 14J. The sheets were then incubated with 10% CO2 at 37°C and samples were collected and analyzed 3 days later, using RT‐PCR and immunofluorescent staining. Results Collagen III expressions significantly increased in both mRNA and protein levels at approximately 12 J. Conclusions There appears to be a threshold effect where there is very little increased collagen III mRNA and protein expression until the laser fluence reaches around 12J. © 2005 Wiley‐Liss, Inc.