Production of alkaline protease from an alkaliphilic actinomycete

• Published in: Bioresource technology 2006-09, Vol.97 (14), p.1650-1654
• Main Authors: Mehta, V.J., Thumar, J.T., Singh, S.P.
• Format: Article
• Language: English
• Subjects: Actinobacteria - classification; Actinobacteria - enzymology; Actinobacteria - isolation & purification; Actinobacteria - metabolism; Agro-industrial by-products; Alkaline protease; Alkaliphilic actinomycete; Amino Acids - metabolism; Amino Acids - pharmacology; Bacterial Proteins - biosynthesis; Bacterial Proteins - isolation & purification; Bacterial Proteins - metabolism; Biological and medical sciences; Bioreactors - microbiology; Biotechnology; Carbohydrate Metabolism; Culture Media - metabolism; Dietary Fiber - metabolism; Dose-Response Relationship, Drug; Endopeptidases - biosynthesis; Endopeptidases - isolation & purification; Endopeptidases - metabolism; Enzyme engineering; Enzyme repression; Fermentation; Flour; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Glucose - pharmacology; Hydrogen-Ion Concentration; Methods. Procedures. Technologies; Molasses; Nitrogen - pharmacology; Peptones - metabolism; Phosphates - metabolism; Phosphates - pharmacology; Production of selected enzymes; Soil Microbiology; Stimulation of enzyme synthesis; Substrate Specificity; Triticum - chemistry; Triticum - metabolism; Triticum aestivum
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2005.07.023

The repression of alkaline protease synthesis from alkaliphilic actinomycete was studied by using glucose, peptone, yeast extract, KH 2PO 4 and amino acids; tyrosine, tryptophan, lysine, and arginine. There was a critical limit of stimulation of enzyme production by these components. Crude components such as molasses, wheat flour, and wheat bran were found to be effective for growth and enzyme production. The high level of enzyme production using agro-industrial by-products is commercially significant due to cheap nature of these sources. The findings are quite attractive, as only few actinomycetes, particularly alkaliphilic ones, have so far been explored for their enzymatic potential and regulation of enzyme synthesis.