Rats Subjected to Extended L-Tryptophan Restriction During Early Postnatal Stage Exhibit Anxious-Depressive Features and Structural Changes

• Published in: Journal of neuropathology and experimental neurology 2006-06, Vol.65 (6), p.562-570
• Main Authors: Zhang, Limei, Guadarrama, Leyla, Corona-Morales, Aleph A, Vega-Gonzalez, Arturo, Rocha, Luisa, Escobar, Alfonso
• Format: Article
• Language: English
• Subjects: Age Factors; Animals; Animals, Newborn; Anxiety - physiopathology; Behavior, Animal; Biological and medical sciences; Brain - metabolism; Brain - ultrastructure; Bromodeoxyuridine - metabolism; Cell Count - methods; Depression - physiopathology; Diet, Protein-Restricted - methods; Disease Models, Animal; Immunohistochemistry - methods; Male; Maze Learning - physiology; Medical sciences; Neurology; Ophthalmology; Proto-Oncogene Proteins c-fos - metabolism; Rats; Rats, Wistar; Retinopathies; Serotonin - metabolism; Silver Staining - methods; Swimming; Tryptophan - deficiency; Tryptophan Hydroxylase - metabolism; Vascular diseases and vascular malformations of the nervous system
• ISSN: 0022-3069; 1554-6578
• DOI: 10.1097/00005072-200606000-00004

Serotonin transmission dysfunction has been suggested to play an important role in depression and anxiety. This study reports the results of a series of experiments in which rats were subjected to extended maize-based tortilla diets during early postnatal stages. This diet contains only approximately 20% of the L-tryptophan in normal diets of laboratory rodents. Compared with controls, experimental rats displayed a significant increase of immobility counts in the forced swimming test and exhibited anxiety-like behavior in the elevated plus maze test after 1 month of diet treatment. Low levels of serotonin contents were found in prefrontal cortex, striatum, hippocampus, and brainstem using high-performance liquid chromatography. Immunocytochemical reactions against 5-Bromo-2′-deoxyuridine revealed a significant decrease in the proliferation rate for the subgranular zone of dentate gyrus. c-Fos expression after the forced swimming test was found reduced in prefrontal cortex, dentate gyrus, CA1, and hilus of hippocampus and amygdala. Moreover, dendrite arbor atrophy and decreased spine density were evident in Golgi-Cox-impregnated CA1 pyramidal neurons. Abnormal dendrite swelling in dentate gyrus granule cells was also observed. These findings indicate an involvement of hyposerotoninergia in emotional disturbance produced by L-tryptophan restriction during critical developmental stages and suggest that neuroplasticity changes might underlie these changes.