Effects of 5 daily injections of the neurotensin-mimetic NT69L on the expression of neurotensin receptors in rat brain

The effects of one or five daily intraperitoneal injections of a neurotensin (NT) receptor agonist NT69L (2 mg/kg, i.p.) on the expression of NT (NTS), dopamine 1 and 2 receptors, tyrosine hydroxylase, and DOPA decarboxylase using immunohistochemical and real-time PCR were investigated in rats. Exce...

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Bibliographic Details
Published in:Brain research. Molecular brain research. 2005-07, Vol.138 (1), p.24-34
Main Authors: Wang, Rui, Boules, Mona, Gollatz, Elisa, Williams, Katrina, Tiner, William, Richelson, Elliott
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Autoradiography - methods
Biological and medical sciences
Brain - anatomy & histology
Brain - drug effects
Brain - metabolism
Cell Count - methods
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Dopa Decarboxylase - genetics
Dopa Decarboxylase - metabolism
Drug Administration Schedule
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation - drug effects
Immunohistochemistry - methods
Male
mRNA
Neurotensin - administration & dosage
Neurotensin - analogs & derivatives
Neurotensin agonist
Neurotensin receptors
Nuclear Receptor Subfamily 4, Group A, Member 2
Peptide Fragments - administration & dosage
Rat
Rats
Rats, Sprague-Dawley
Receptors, Dopamine - genetics
Receptors, Dopamine - metabolism
Receptors, Neurotensin - classification
Receptors, Neurotensin - genetics
Receptors, Neurotensin - metabolism
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - biosynthesis
Tolerance
Transcription Factors - genetics
Transcription Factors - metabolism
Tyrosine 3-Monooxygenase - metabolism
Tyrosine hydroxylase
Vertebrates: nervous system and sense organs
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Summary:The effects of one or five daily intraperitoneal injections of a neurotensin (NT) receptor agonist NT69L (2 mg/kg, i.p.) on the expression of NT (NTS), dopamine 1 and 2 receptors, tyrosine hydroxylase, and DOPA decarboxylase using immunohistochemical and real-time PCR were investigated in rats. Except for the striatum, acute injection of NT69L did not affect neurotensin receptors as compared to saline control. However, 5 daily injections of NT69L resulted in down-regulation of both NTS-1 protein and mRNA levels in several brain regions with the striatum showing a dramatic decrease in NTS-1 expression ( P 
ISSN:0169-328X
1872-6941
DOI:10.1016/j.molbrainres.2005.03.014