The prevalence of human papillomavirus (HPV) infection in paired urine and cervical smear samples of HIV-infected women

• Published in: Journal of clinical virology 2008-02, Vol.41 (2), p.111-115
• Main Authors: Jong, Eefje, Mulder, Jan Willem, van Gorp, Eric C.M, Wagenaar, Jiri K.S, Derksen, Joke, Westerga, Johan, Tol, Anja, Smits, Paul H.M
• Format: Article
• Language: English
• Subjects: Adult; Allergy and Immunology; Biological and medical sciences; Cervical scrapes; Cervix Uteri - virology; DNA, Viral - analysis; DNA, Viral - isolation & purification; Epidemiology; Female; Fundamental and applied biological sciences. Psychology; HIV; HIV Infections - complications; Human immunodeficiency virus; Human papillomavirus; Human viral diseases; Humans; Infectious Disease; Infectious diseases; Medical sciences; Microbiology; Miscellaneous; Papanicolaou Test; Papillomaviridae - classification; Papillomaviridae - genetics; Papillomaviridae - isolation & purification; Papillomavirus Infections - complications; Papillomavirus Infections - epidemiology; Papillomavirus Infections - virology; PCR; Prevalence; Urine; Urine - virology; Vaginal Smears; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Virology; Women
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2007.10.007

Abstract Background HIV-infected women are at increased risk for cervical dysplasia. Cervical dysplasia is caused by persistent infections with certain types of human papillomavirus (HPV). Conventional testing for genital HPV infections requires cervical cytology. A non-invasive screening method by detection of HPV DNA in urine samples is preferable but is not a routine practice. Objectives To investigate the prevalence and concordance of HPV in paired urine and cervical smear samples and cytological results of Pap smears in HIV-infected women. Study design Paired urine and cervical smear samples were collected from 27 HIV-infected women. Results The HPV prevalence in urine and cervical smear samples was 81.5% and 51.9%, respectively ( p = 0.01). The concordance for HPV positivity and negativity between urine and cervical smear samples is 71%. Seven women (25.9%) had an abnormal cervical smear of Pap II or higher. In all urine samples from these cases HPV DNA was detected. Conclusion In the present study we show that the HPV prevalence in urine and cervical smear samples of HIV-infected women is high and HPV test results are highly concordant. Therefore, urine samples can be used as medium for HPV testing. HPV testing in urine samples is a simple, reliable, non-invasive screening method.