Rat Schwann cells express M1-M4 muscarinic receptor subtypes

The expression of different muscarinic receptor subtypes was analyzed in immature Schwann cells obtained from sciatic nerve of 2‐day neonatal rats. By using RT‐PCR analysis, we demonstrated the presence of M1, M2, M3, and M4 receptor subtypes in cultured Schwann cells, with M2 displaying the highest...

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Bibliographic Details
Published in:Journal of neuroscience research 2006-07, Vol.84 (1), p.97-105
Main Authors: Loreti, Simona, Vilaró, M. Teresa, Visentin, S., Rees, H., Levey, Allan I., Tata, Ada Maria
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Animals, Newborn
Blotting, Northern - methods
Calcium - metabolism
Cells, Cultured
Colforsin - pharmacology
development
Gene Expression - drug effects
Gene Expression - physiology
Immunoprecipitation - methods
In Situ Hybridization - methods
Inositol 1,4,5-Trisphosphate - metabolism
muscarinic acetylcholine receptors
Rats
Rats, Wistar
Receptors, Muscarinic - classification
Receptors, Muscarinic - genetics
Receptors, Muscarinic - metabolism
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - metabolism
Schwann Cells - drug effects
Schwann Cells - metabolism
Sciatic Nerve - cytology
signal transduction
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Summary:The expression of different muscarinic receptor subtypes was analyzed in immature Schwann cells obtained from sciatic nerve of 2‐day neonatal rats. By using RT‐PCR analysis, we demonstrated the presence of M1, M2, M3, and M4 receptor subtypes in cultured Schwann cells, with M2 displaying the highest expression levels. Muscarinic subtypes were also quantified by immunoprecipitation and [3H]QNB binding. With this approach, we found the levels of receptor expression to be M2 > M3 > M1. M4 is expressed at very low levels, and M5 receptor was not detectable. Moreover, we also demonstrated that stimulation of the receptors by muscarinic agonists activates previously described signal transduction pathways, leading to a decrease of cAMP and an increase of IP3 levels not associated with an efficient intracellular Ca2+ release. The presence and activity of particular muscarinic receptors in immature Schwann cells suggest that ACh may play an important role in Schwann cell development. © 2006 Wiley‐Liss, Inc.
ISSN:0360-4012
1097-4547
DOI:10.1002/jnr.20874