Endothelin-1 activates Homer 1alpha expression via mitogen-activated protein kinase in cardiac myocytes

Homer proteins are a family of scaffolding proteins which may play an important role in calcium signaling by facilitating the assembly of signaling complexes in neuronal cells. Among the three splice variants of Homer 1, Homer 1alpha is rapidly up-regulated by neural stimulation and may regulate the...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular medicine 2006-07, Vol.18 (1), p.193-196
Main Authors: Kawamoto, Takahiro, Togi, Kiyonori, Yamauchi, Ryoko, Yoshida, Yoshinori, Nakashima, Yasuhiro, Kita, Toru, Tanaka, Makoto
Format: Article
Language:English
Subjects:
Angiotensin II - pharmacology
Animals
Animals, Newborn
Blotting, Northern
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cells, Cultured
Endothelin-1 - pharmacology
Enzyme Activation - drug effects
Enzyme Inhibitors - pharmacology
Flavonoids - pharmacology
Gene Expression - drug effects
Homer Scaffolding Proteins
Imidazoles - pharmacology
Mitogen-Activated Protein Kinases - antagonists & inhibitors
Mitogen-Activated Protein Kinases - metabolism
Myocytes, Cardiac - drug effects
Myocytes, Cardiac - metabolism
Protein Isoforms - genetics
Protein Isoforms - metabolism
Pyridines - pharmacology
Rats
Rats, Sprague-Dawley
RNA, Messenger - genetics
RNA, Messenger - metabolism
Signal Transduction - drug effects
Time Factors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Homer proteins are a family of scaffolding proteins which may play an important role in calcium signaling by facilitating the assembly of signaling complexes in neuronal cells. Among the three splice variants of Homer 1, Homer 1alpha is rapidly up-regulated by neural stimulation and may regulate the disassembly of signaling complexes mediated by Homer proteins. In spite of its potential importance in calcium signaling, the regulation of Homer 1alpha expression in cardiac myocytes has never been investigated. In this study, we examined the regulation of Homer 1alpha expression in cardiac myocytes. Homer 1alpha was significantly up-regulated by several hypertrophic agonists, including endothelin-1 (ET-1), phenylephrine, isoprotenerol and angiotensin-II, and ET-1 most strikingly induced Homer 1alpha expression. The induction of Homer 1alpha expression by ET-1 peaked at 2 h and inhibitors for mitogen-activated/extracellular signal regulated kinase (MEK) significantly suppressed the induction of Homer 1alpha. This study first clarified the regulation of Homer 1alpha expression in cardiac myocytes and demonstrated that ET-1 induced Homer 1alpha expression through the mitogen-activated protein kinase pathway.
ISSN:1107-3756