Simultaneous Determination of Eight Water-Soluble Vitamins in Supplemented Foods by Liquid Chromatography

A fast, simple, and reliable method for the isolation and determination of the vitamins thiamin, riboflavin, niacin, pantothenic acid, pyridoxine, folic acid, cyanocobalamin, and ascorbic acid in food samples is proposed. The most relevant advantages of the proposed method are the simultaneous deter...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2006-06, Vol.54 (13), p.4531-4536
Main Authors: Zafra-Gómez, Alberto, Garballo, Antonio, Morales, Juan C, García-Ayuso, Luis E
Format: Article
Language:English
Subjects:
Animals
ascorbic acid
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
cyanocobalamin
enriched foods
folic acid
food analysis
Food industries
Food, Fortified - analysis
Fundamental and applied biological sciences. Psychology
high performance liquid chromatography
Infant Formula - chemistry
infant formulas
milk
Milk - chemistry
Milk and cheese industries. Ice creams
niacin
pantothenic acid
pyridoxine
Reference Standards
Reproducibility of Results
Research and development. New food products, dietetic foods and beverages
riboflavin
Solubility
thiamin
vitamin content
Vitamins - analysis
Water
water-soluble vitamins
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Summary:A fast, simple, and reliable method for the isolation and determination of the vitamins thiamin, riboflavin, niacin, pantothenic acid, pyridoxine, folic acid, cyanocobalamin, and ascorbic acid in food samples is proposed. The most relevant advantages of the proposed method are the simultaneous determination of the eight more common vitamins in enriched food products and a reduction of the time required for quantitative extraction, because the method consists merely of the addition of a precipitation solution and centrifugation of the sample. Furthermore, this method saves a substantial amount of reagents as compared with official methods, and minimal sample manipulation is achieved due to the few steps required. The chromatographic separation is carried out on a reverse phase C18 column, and the vitamins are detected at different wavelengths by either fluorescence or UV−visible detection. The proposed method was applied to the determination of water-soluble vitamins in supplemented milk, infant nutrition products, and milk powder certified reference material (CRM 421, BCR) with recoveries ranging from 90 to 100%. Keywords: Water-soluble vitamins; food labeling; supplemented foods; milk; liquid chromatography
ISSN:0021-8561
1520-5118
DOI:10.1021/jf060346e