Comparative analysis of mu-opioid receptor expression in immune and neuronal cells

Abstract Morphine modulates neuronal and immune cell functions via μ-opioid receptors. In primary and Jurkat T cells, and Raji B cells μ-opioid receptor transcripts were detected only after stimulation of the cells with IL-4 or TNF-α. Moreover, the amount of the induced μ-opioid receptor mRNA in the...

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Bibliographic Details
Published in:Journal of neuroimmunology 2007-08, Vol.188 (1), p.56-63
Main Authors: Börner, Christine, Stumm, Ralf, Höllt, Volker, Kraus, Jürgen
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Cells, Cultured
Cerebral Cortex - cytology
Cyclic AMP - metabolism
Embryo, Mammalian
Gene Expression Regulation - immunology
Gene Expression Regulation - physiology
Glutamate Decarboxylase - genetics
Glutamate Decarboxylase - metabolism
Humans
In Situ Hybridization
Interleukin-4
Interleukin-4 - pharmacology
Lymphocyte
Mitogen activated protein kinase
Mitogen-Activated Protein Kinases - genetics
Mitogen-Activated Protein Kinases - metabolism
Morphine - pharmacology
Narcotics - pharmacology
Neuroblastoma
Neurology
Neuron
Neurons - metabolism
Rats
Receptors, Opioid, mu - genetics
Receptors, Opioid, mu - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Somatostatin - analogs & derivatives
Somatostatin - pharmacology
T-Lymphocytes - drug effects
T-Lymphocytes - metabolism
Tumor necrosis factor
Tumor Necrosis Factor-alpha - pharmacology
Vesicular Glutamate Transport Protein 1 - genetics
Vesicular Glutamate Transport Protein 1 - metabolism
μ-Opioid receptor
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Summary:Abstract Morphine modulates neuronal and immune cell functions via μ-opioid receptors. In primary and Jurkat T cells, and Raji B cells μ-opioid receptor transcripts were detected only after stimulation of the cells with IL-4 or TNF-α. Moreover, the amount of the induced μ-opioid receptor mRNA in the immune cells was 15 to 200 times less than those in primary cortical and SH SY5Y neuronal cells. Nevertheless, μ-opioid receptor mRNA in immune cells is processed to functional receptors, as demonstrated by morphine-mediated phosphorylation of mitogen activated protein kinase, morphine-mediated up-regulation of IL-4 mRNA and coupling to adenylyl cyclase in Jurkat cells.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2007.05.007