Intranasal immunization with influenza virus and Korean mistletoe lectin C (KML-C) induces heterosubtypic immunity in mice

Abstract The mucosal adjuvanticity of Korean mistletoe lectin C (KML-C) was investigated in mice intranasally immunized with inactivated influenza virus (H1N1). Mucosal and systemic immune responses were compared to those induced with cholera toxin B subunit (CTB). KML-C increased influenza-specific...

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Bibliographic Details
Published in:Vaccine 2007-08, Vol.25 (34), p.6359-6366
Main Authors: Song, Seong Kyu, Moldoveanu, Zina, Nguyen, Huan H, Kim, Eui Ho, Choi, Kwan Yong, Kim, Jong Bae, Mestecky, Jiri
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - administration & dosage
Administration, Intranasal
Allergy and Immunology
Animals
Antibodies, Viral - biosynthesis
Applied microbiology
Biological and medical sciences
Female
Fundamental and applied biological sciences. Psychology
Heterosubtypic immunity
Immune system
Immunization
Influenza
Influenza A Virus, H1N1 Subtype - immunology
Influenza A Virus, H3N2 Subtype - immunology
Influenza Vaccines - administration & dosage
Influenza virus
Korean mistletoe lectin
Lymph nodes
Lymphocyte Activation
Lymphocytes
Medical research
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Mistletoe
Mucosal adjuvant
Oligodeoxyribonucleotides - administration & dosage
Plant Lectins - administration & dosage
Rodents
Santalales
T-Lymphocytes, Cytotoxic - immunology
Toxins
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Virology
Waterborne diseases
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Summary:Abstract The mucosal adjuvanticity of Korean mistletoe lectin C (KML-C) was investigated in mice intranasally immunized with inactivated influenza virus (H1N1). Mucosal and systemic immune responses were compared to those induced with cholera toxin B subunit (CTB). KML-C increased influenza-specific antibodies with dominant IgG1 subclass in serum, IgG in genital secretions and IgA in saliva, and significantly enhanced influenza-specific lymphocyte proliferation and cytotoxic activity in spleens and in mediastinal lymph nodes. When KML-C was used as a mucosal adjuvant, mice were completely protected from mortality after the challenge with a homologous (H1N1) mouse-adapted influenza virus. After challenge with heterologous (H3N2) influenza virus the level of heterosubtypic immunity in KML-C-treated mice was comparable to that of mice that received CTB as adjuvant. These findings suggest that KML-C may be used as an effective mucosal adjuvant.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2007.06.030