Expression profiling of auxin-treated Arabidopsis roots: Toward a molecular analysis of lateral root emergence

Treating Arabidopsis roots with exogenous auxin results in dramatic changes in cellular processes including de novo induction of lateral roots which later emerge through the overlying cells. Microarray experiments reveal approximately 80 genes that are substantially up-regulated in the root over the...

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Bibliographic Details
Published in:Plant and cell physiology 2006-06, Vol.47 (6), p.788-792
Main Authors: Laskowski, M.(Oberlin Coll., Ohio (USA)), Biller, S, Stanley, K, Kajstura, T, Prusty, R
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - physiology
Arabidopsis Proteins - analysis
Arabidopsis Proteins - genetics
Arabidopsis Proteins - physiology
ARABIDOPSIS THALIANA
Auxin
AUXINAS
AUXINE
AUXINS
Cell separation
Cell Wall - drug effects
Cell Wall - physiology
DNA, Plant - analysis
DNA, Plant - genetics
EST
EXPRESION GENICA
expressed sequence tag
EXPRESSION DES GENES
GENE
GENE EXPRESSION
Gene Expression Profiling
Gene Expression Regulation, Plant - drug effects
Gene Expression Regulation, Plant - genetics
Gene Expression Regulation, Plant - physiology
GENES
Genes, Plant - genetics
Genes, Plant - physiology
Indoleacetic Acids - pharmacology
Lateral root
Methylation
Microarray
Oligonucleotide Array Sequence Analysis
Pectate lyase
pectin methyl esterase
PECTINAS
PECTINE
PECTINS
Phospholipases A - analysis
Phospholipases A - genetics
Phospholipases A - physiology
Plant Roots - chemistry
Plant Roots - drug effects
Plant Roots - growth & development
PME
polygalacturonase
Polysaccharide-Lyases - analysis
Polysaccharide-Lyases - genetics
Polysaccharide-Lyases - physiology
qPCR
quantitative real-time PCR
RACINE
RAICES
ROOTS
scanning electron microscopy
SEM
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Description
Summary:Treating Arabidopsis roots with exogenous auxin results in dramatic changes in cellular processes including de novo induction of lateral roots which later emerge through the overlying cells. Microarray experiments reveal approximately 80 genes that are substantially up-regulated in the root over the first 12 h following auxin treatment. We hypothesize that the observed increase in expression of pectate lyase family genes leads to degradation of the pectin-rich middle lamellae, allowing cells in the parent root to separate cleanly. Differences in the degree of pectin methylation in lateral and parent roots may explain why lateral roots are not degraded themselves.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcj043