Effect of oocyte mitochondrial DNA haplotype on bovine somatic cell nuclear transfer efficiency

The development capability of reconstructed bovine embryos via ovum pick‐up (OPU)‐somatic cell nuclear transfer (SCNT) technique has been influenced by the maternal lineage of oocyte cytoplasm, but the underlying mechanism remains unclear. Since mitochondria are the richest maternal‐inherited organe...

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Bibliographic Details
Published in:Molecular reproduction and development 2007-10, Vol.74 (10), p.1278-1286
Main Authors: Jiao, Fei, Yan, Jing-Bin, Yang, Xiao-Yu, Li, Hua, Wang, Qingxue, Huang, Shu-Zhen, Zeng, Fanyi, Zeng, Yi-Tao
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - analysis
Animals
ATP
Biological and medical sciences
Cattle
DNA, Mitochondrial - physiology
Efficiency
Embryonic Development - genetics
Female
Fundamental and applied biological sciences. Psychology
Gene expression
haplotype
Haplotypes - physiology
mitochondrial DNA
Molecular and cellular biology
Molecular genetics
Nuclear Transfer Techniques
Oocytes - chemistry
Oocytes - metabolism
OPU
PCR-RFLP
Prostaglandin-Endoperoxide Synthases - analysis
Prostaglandin-Endoperoxide Synthases - genetics
Protein Subunits - analysis
Protein Subunits - genetics
SCNT
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Summary:The development capability of reconstructed bovine embryos via ovum pick‐up (OPU)‐somatic cell nuclear transfer (SCNT) technique has been influenced by the maternal lineage of oocyte cytoplasm, but the underlying mechanism remains unclear. Since mitochondria are the richest maternal‐inherited organelle, in this study, we intended to clarify the effect of mtDNA haplotypes on cloning efficiency. By PCR‐RFLP method, we identified mtDNA haplotypes A and B, differing in six restriction sites. Reconstructed embryos with haplotype A cytoplast achieved better fusion and blastocyst formation rate (64.6% and 39.4%), as compared with haplotype B (53.6% and 26.3%; P 
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.20698