Characterization of Biomarkers in Polycystic Ovary Syndrome (PCOS) Using Multiple Distinct Proteomic Platforms

A variety of prefractionation methods (including a novel reversed-phase solid-phase-extraction (RP-SPE) combined with SDS-PAGE and proteomic based approaches (e.g., 2-dimensional gel electrophoresis (2DE) and MALDI-TOF mass spectrometry combined with Artificial Neural Network (ANN) bioinformatic too...

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Bibliographic Details
Published in:Journal of proteome research 2007-08, Vol.6 (8), p.3321-3328
Main Authors: Matharoo-Ball, B, Hughes, C, Lancashire, L, Tooth, D, Ball, G, Creaser, C, Elgasim, M, Rees, R, Layfield, R, Atiomo, W
Format: Article
Language:English
Subjects:
Biomarkers - metabolism
Electrophoresis, Gel, Two-Dimensional - methods
Female
Humans
Polycystic Ovary Syndrome - metabolism
Proteome - metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:A variety of prefractionation methods (including a novel reversed-phase solid-phase-extraction (RP-SPE) combined with SDS-PAGE and proteomic based approaches (e.g., 2-dimensional gel electrophoresis (2DE) and MALDI-TOF mass spectrometry combined with Artificial Neural Network (ANN) bioinformatic tools) were used to investigate the protein/peptide signatures in patients with Polycystic Ovary Syndrome (PCOS). Four potential PCOS biomarkers were identified (complement C4α3c and C4γ and haptoglobin α and β chains). Keywords: Polycystic Ovary Syndrome • 2DE • MALDI-TOF MS • Complement Proteins • Haptoglobin
ISSN:1535-3893
1535-3907
DOI:10.1021/pr070124b