Two SCARECROW-LIKE genes are induced in response to exogenous auxin in rooting-competent cuttings of distantly related forest species

We characterized SCARECROW-LIKE genes induced by auxin in rooting-competent cuttings of two distantly related forest species (Pinus radiata D. Don and Castanea sativa Mill.) before the activation of cell division that results in adventitious root formation. The predicted protein sequences contain do...

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Bibliographic Details
Published in:Tree physiology 2007-10, Vol.27 (10), p.1459-1470
Main Authors: Sanchez, C, Vielba, J.M, Ferro, E, Covelo, G, Sole, A, Abarca, D, DeMier, B.S. de, Diaz-Sala, C
Format: Article
Language:English
Subjects:
adventitious roots
Amino Acid Sequence
amino acid sequences
Arabidopsis Proteins
auxins
Base Sequence
Castanea sativa
Fagaceae - drug effects
Fagaceae - genetics
Fagaceae - metabolism
forest trees
gene expression
Gene Expression Regulation, Plant - drug effects
genes
Genes, Plant - genetics
Indoleacetic Acids - pharmacology
Molecular Sequence Data
nucleotide sequences
Phylogeny
Pinus - drug effects
Pinus - genetics
Pinus - metabolism
Pinus radiata
Plant Components, Aerial - genetics
Plant Components, Aerial - metabolism
plant cuttings
Plant Proteins - biosynthesis
Plant Proteins - genetics
Plant Roots - genetics
Plant Roots - metabolism
root growth
root meristems
rooting
Trees
vegetative propagation
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Summary:We characterized SCARECROW-LIKE genes induced by auxin in rooting-competent cuttings of two distantly related forest species (Pinus radiata D. Don and Castanea sativa Mill.) before the activation of cell division that results in adventitious root formation. The predicted protein sequences contain domains characteristic of the GRAS protein family and show a strong similarity to the SCARECROW-LIKE proteins, indicating conserved functions of these proteins. Quantitative RT-PCR analysis showed that these genes are expressed at relatively high levels in roots. Induction of increased mRNA levels in rooting-competent cuttings of both species in response to exogenous auxin was observed within the first 24 h of the root induction process, a time when cell reorganization takes place, but before the resumption of cell division and the appearance of adventitious root primordia. These results suggest that SCARECROW-LIKE genes play a role during the earliest stages of adventitious root formation.
ISSN:0829-318X
1758-4469
DOI:10.1093/treephys/27.10.1459