Structure of a new ribitol teichoic acid-like O-polysaccharide of a serologically separate Proteus vulgaris strain, TG 276-1, classified into a new Proteus serogroup O53

where the non-glycosylated ribitol residue is randomly mono-O-acetylated. An unusual ribitol teichoic acid-like O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide from a previously non-classified Proteus vulgaris strain TG 276-1. Structural studies using chemical analys...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate research 2007-10, Vol.342 (14), p.2061-2066
Main Authors: Arbatsky, Nikolay P., Kondakova, Anna N., Senchenkova, Sof’ya N., Siwińska, Malgorzata, Shashkov, Alexander S., Zych, Krystyna, Knirel, Yuriy A., Sidorczyk, Zygmunt
Format: Article
Language:English
Subjects:
Deamination
Lipopolysaccharides - chemistry
Magnetic Resonance Spectroscopy
Molecular Structure
NMR spectroscopy
O Antigens
Proteus - classification
Proteus vulgaris
Proteus vulgaris - chemistry
Proteus vulgaris - classification
Ribitol phosphate
Serological classification
Teichoic Acids
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:where the non-glycosylated ribitol residue is randomly mono-O-acetylated. An unusual ribitol teichoic acid-like O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide from a previously non-classified Proteus vulgaris strain TG 276-1. Structural studies using chemical analyses and 2D 1H and 13C NMR spectroscopy showed that the polysaccharide is a zwitterionic polymer with a repeating unit containing 2-acetamido-4-amino-2,4,6-trideoxy- d-galactose ( d-FucNAc4N) and two d-ribitol phosphate ( d-Rib-ol-5- P) residues and having the following structure: [Display omitted] where the non-glycosylated ribitol residue is randomly mono-O-acetylated. Based on the unique O-polysaccharide structure and the finding that the strain studied is serologically separate among Proteus bacteria, we propose to classify P. vulgaris strain TG 276-1 into a new Proteus serogroup, O53.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2007.05.036