Validation of the indirect fluorescent antibody and the complement fixation tests for the diagnosis of Theileria equi

The indirect fluorescent antibody (IFA) test for Theileria equi was evaluated to assess test's suitability for the serological diagnosis of equine piroplasmosis, to provide performance parameters for the purpose of test validation, and to compare it with the complement fixation (CF) test. Using...

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Bibliographic Details
Published in:Veterinary parasitology 2007-09, Vol.148 (2), p.102-108
Main Authors: Ogunremi, Oladele, Georgiadis, Marios P., Halbert, Gary, Benjamin, Jane, Pfister, Kurt, Lopez-Rebollar, Laura
Format: Article
Language:English
Subjects:
Animals
antibody detection
Bayes Theorem
Bayesian
Bayesian theory
complement fixation tests
Complement Fixation Tests - methods
Complement Fixation Tests - standards
Complement Fixation Tests - veterinary
Diagnosis, Differential
diagnostic techniques
equine piroplasmosis
fluorescent antibody technique
Fluorescent Antibody Technique, Indirect - methods
Fluorescent Antibody Technique, Indirect - standards
Fluorescent Antibody Technique, Indirect - veterinary
Horse Diseases - diagnosis
Horses
IFA
Reproducibility of Results
Sensitivity
Sensitivity and Specificity
serodiagnosis
Specificity
statistical analysis
test sensitivity
test specificity
Theileria - immunology
Theileria equi
Theileriasis - diagnosis
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Summary:The indirect fluorescent antibody (IFA) test for Theileria equi was evaluated to assess test's suitability for the serological diagnosis of equine piroplasmosis, to provide performance parameters for the purpose of test validation, and to compare it with the complement fixation (CF) test. Using a protocol that included Evan's blue, the specificity of the IFA test was estimated at 99.0% for T. equi by the classical method of analysis, and 96.6% by the Bayesian method. The use of Evan's blue in the test protocol increased test specificity and contributed to an excellent test agreement between two collaborating laboratories (kappa = 0.96). Using Bayesian analysis, the sensitivity estimate for the IFA test was 89.2%. The CF test sensitivity and specificity estimates for T. equi were 63.1 and 96.4%, respectively, as determined by Bayesian analysis. The IFA test was more sensitive than the CF test but the specificity estimates were similar.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2007.06.006