Microtubule-Severing Activity of Shigella Is Pivotal for Intercellular Spreading

Some pathogenic bacteria actually invade the cytoplasm of their target host cells. Invasive bacteria acquire the propulsive force to move by recruiting actin and inducing its polymerization. Here we show that Shigella movement within the cytoplasm was severely hindered by microtubules and that the b...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2006-11, Vol.314 (5801), p.985-989
Main Authors: Yoshida, Sei, Handa, Yutaka, Suzuki, Toshihiko, Ogawa, Michinaga, Suzuki, Masato, Tamai, Asuka, Abe, Akio, Katayama, Eisaku, Sasakawa, Chihiro
Format: Article
Language:English
Subjects:
Actins
Actins - metabolism
Animals
Antibodies
Bacteria
Bacteriology
Biological and medical sciences
Cell adhesion & migration
Cercopithecus aethiops
COS Cells
Cysteine Endopeptidases - metabolism
Cytoplasm
Cytoplasm - microbiology
Dysentery, Bacillary - microbiology
Epithelial cells
Fundamental and applied biological sciences. Psychology
Immunoblotting
Mice
Mice, Inbred C57BL
Microbiology
Microscopy, Fluorescence
Microtubules
Microtubules - drug effects
Microtubules - metabolism
Microtubules - ultrastructure
Movement
Mutation
Nocodazole - pharmacology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Polymerization
Proteases
Shigella
Shigella flexneri - enzymology
Shigella flexneri - genetics
Shigella flexneri - pathogenicity
Shigella flexneri - physiology
Tubulin - metabolism
Virulence Factors - metabolism
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Description
Summary:Some pathogenic bacteria actually invade the cytoplasm of their target host cells. Invasive bacteria acquire the propulsive force to move by recruiting actin and inducing its polymerization. Here we show that Shigella movement within the cytoplasm was severely hindered by microtubules and that the bacteria destroyed surrounding microtubules by secreting VirA by means of the type III secretion system. Degradation of microtubules by VirA was dependent on its α-tubulin-specific cysteine protease-like activity. virA mutants did not move within the host cytoplasm and failed to move into adjacent cells.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1133174