Validation of an HPLC method for analysis of DB-67 and its water soluble prodrug in mouse plasma

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2006-11, Vol.844 (1), p.15-22
• Main Authors: Horn, Jamie, Jordan, Sherri L., Song, Lin, Roberts, Michael J., Anderson, Bradley D., Leggas, Markos
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Camptothecin - analogs & derivatives; Camptothecin - blood; Camptothecin - pharmacokinetics; Camptothecin analogs; Chromatography, High Pressure Liquid - methods; DB-67; Fundamental and applied biological sciences. Psychology; General pharmacology; Male; Medical sciences; Method validation; Mice; Mice, Inbred C57BL; Organosilicon Compounds - blood; Organosilicon Compounds - pharmacokinetics; Pharmacology. Drug treatments; Prodrugs - pharmacokinetics; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Fluorescence
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2006.06.022

A method for the quantitation of DB-67 ((20 S)-10-hydroxy-7- tert-butyldimethylsilylcamptothecin) lactone and carboxylate in mouse plasma has been developed, validated, and applied in pharmacokinetic studies. The analytes were separated by reversed-phase chromatography with fluorescence detection. Validation demonstrated the selectivity and specificity for the carboxylate and lactone, with linearity between 1–300 ng/mL and 2.5–300 ng/mL for the carboxylate and lactone, respectively (accuracy 90–110% of theory and coefficient of variation ≤5.7%). Carboxylate to lactone conversion was