Electrophoretically mediated microanalysis of a nicotinamide adenine dinucleotide-dependent enzyme and its facile multiplexing using an active pixel sensor UV detector

An electrophoretically mediated microanalysis (EMMA) method has been developed for yeast alcohol dehydrogenase and quantification of reactant and product cofactors, NAD and NADH. The enzyme substrate ethanol (1% (v/v)) was added to the buffer (50 mM borate, pH 8.8). Results are presented for paralle...

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Bibliographic Details
Published in:Journal of Chromatography A 2007-08, Vol.1162 (2), p.132-140
Main Authors: Urban, Pawel L., Goodall, David M., Bergström, Edmund T., Bruce, Neil C.
Format: Article
Language:English
Subjects:
Active pixel sensor
Alcohol Dehydrogenase - analysis
Alcohol Dehydrogenase - metabolism
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Capillary Electrochromatography - instrumentation
Capillary Electrochromatography - methods
Capillary electrophoresis
EMMA
Enzyme assay
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Isoenzymes - analysis
Isoenzymes - metabolism
Microchemistry - instrumentation
Microchemistry - methods
NAD
NAD - metabolism
Oxidoreductases
Plug-plug
Reproducibility of Results
Spectrophotometry, Ultraviolet - methods
Time Factors
Yeasts - enzymology
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Summary:An electrophoretically mediated microanalysis (EMMA) method has been developed for yeast alcohol dehydrogenase and quantification of reactant and product cofactors, NAD and NADH. The enzyme substrate ethanol (1% (v/v)) was added to the buffer (50 mM borate, pH 8.8). Results are presented for parallel capillary electrophoresis with a novel miniature UV area detector, with an active pixel sensor imaging an array of two or six parallel capillaries connected via a manifold to a single output capillary in a commercial CE instrument, allowing conversions with five different yeast alcohol dehydrogenase concentrations to be quantified in a single experiment.
ISSN:0021-9673
DOI:10.1016/j.chroma.2007.04.071