HobA - a novel protein involved in initiation of chromosomal replication in Helicobacter pylori

Replication of the bacterial chromosome is initiated by the binding of the DnaA protein to a unique DNA region, called oriC. Many regulatory factors in numerous species act by controlling the ability of DnaA to bind and unwind DNA, but the Helicobacter pylori genome does not contain homologues to an...

Full description

Saved in:
Bibliographic Details
Published in:Molecular microbiology 2007-08, Vol.65 (4), p.979-994
Main Authors: Zawilak-Pawlik, Anna, Kois, Agnieszka, Stingl, Kerstin, Boneca, Ivo G, Skrobuk, Paulina, Piotr, Jakimowicz, Lurz, Rudi, Zakrzewska-Czerwińska, Jolanta, Labigne, Agnés
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
Bacteriology
Base Sequence
Biochemistry
Biological and medical sciences
Chromosomes
Chromosomes, Bacterial - genetics
Conserved Sequence
Deoxyribonucleic acid
DNA
DNA Replication
DNA, Bacterial - metabolism
DNA-Binding Proteins - metabolism
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Genes, Essential
Genomics
Helicobacter pylori
Helicobacter pylori - genetics
Helicobacter pylori - growth & development
Microbiology
Miscellaneous
Protein Binding
Protein Structure, Quaternary
Proteins
Proteobacteria
Replication Origin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Replication of the bacterial chromosome is initiated by the binding of the DnaA protein to a unique DNA region, called oriC. Many regulatory factors in numerous species act by controlling the ability of DnaA to bind and unwind DNA, but the Helicobacter pylori genome does not contain homologues to any of these factors. Here, we describe HobA, a novel protein essential for initiation of H. pylori chromosome replication, which is conserved among, and unique to, epsilon proteobacteria. We demonstrate that HobA interacts specifically via DnaA with the oriC-DnaA complex. We postulate that HobA is essential for correct formation and stabilization of the orisome by facilitating the spatial positioning of DnaA at oriC. Consistent with its function, overexpression of hobA had no effect on growth of H. pylori, whereas depletion of HobA led to growth arrest and failure to initiate replication. In conclusion, HobA may be the first identified of a new group of initiation factors common to epsilon proteobacteria.
ISSN:0950-382X
1365-2958
DOI:10.1111/j.1365-2958.2007.05853.x