DHEA Reduces NGF-Mediated Cell Survival in Serum-Deprived PC12 Cells

:  Adrenocortical androgens, including dehydroepiandrosterone (DHEA), are produced in the inner zone of the adrenal cortex that is in direct contact with the neural crest–derived catecholamine‐producing chromaffin cells. DHEA has recently been identified as a crucial regulator of neuronal stem cell...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2006-08, Vol.1073 (1), p.306-311
Main Authors: ZIEGLER, CHRISTIAN G., SICARD, FLAVIE, SPERBER, SIMONE, EHRHART-BORNSTEIN, MONIKA, BORNSTEIN, STEFAN R., KRUG, ALEXANDER W.
Format: Article
Language:English
Subjects:
Animals
Cell Survival - drug effects
Culture Media, Serum-Free
dehydroepiandrosterone (DHEA)
Dehydroepiandrosterone - pharmacology
Enzyme Activation
Enzyme-Linked Immunosorbent Assay
Mitogen-Activated Protein Kinase 1 - metabolism
Mitogen-Activated Protein Kinase 3 - metabolism
mitogen-activated protein kinases (MAPK)
nerve growth factor (NGF)
Nerve Growth Factor - pharmacology
PC12 Cells
Rats
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Summary::  Adrenocortical androgens, including dehydroepiandrosterone (DHEA), are produced in the inner zone of the adrenal cortex that is in direct contact with the neural crest–derived catecholamine‐producing chromaffin cells. DHEA has recently been identified as a crucial regulator of neuronal stem cell proliferation. Thus, DHEA might play a hitherto unknown role in intra‐adrenal tissue formation. In the present study, we examined the influence of DHEA on nerve growth factor (NGF)–mediated survival in serum‐deprived PC12 cells and analyzed the influence of DHEA on NGF‐induced ERK1/2 mitogen‐activated protein (MAP) kinase activation by enzyme‐linked immunosorbent assay (ELISA). Cell survival promoted by NGF in serum‐deprived PC12 cells and neurite outgrowth was reduced by DHEA, pointing toward a role of DHEA in the differentiation process of chromaffin cells. Furthermore, NGF‐induced ERK 1/2 activation was significantly inhibited by DHEA. Hence, we speculate that DHEA might influence NGF‐mediated chromaffin differentiation processes using the ERK1/2 MAP kinase pathway during adrenal tissue development.
ISSN:0077-8923
1749-6632
DOI:10.1196/annals.1353.035