Cloning and sequencing of the class A β-lactamase gene (blaTEM-15) located on a chromosomal Tn801 transposon

Escherichia coli CA0210 was identified in a stool culture of a 03-month-neonate in Tunisia. This strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefpirome, ceftazidime, and cefotaxime, but it remained susceptible to imipenem and cefoxitin. The beta-lac...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2007-08, Vol.58 (4), p.459-463
Main Authors: CHOUCHANI, Chedly, BEN-ACHOUR, Nahed, M'CHAREK, Arij, BELHADJ, Omrane
Format: Article
Language:English
Subjects:
Bacteriology
Base Sequence
beta-Lactam Resistance - genetics
beta-Lactamases - chemistry
beta-Lactamases - genetics
beta-Lactams - pharmacology
Biological and medical sciences
Cloning, Molecular
DNA Transposable Elements
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - genetics
Escherichia coli - isolation & purification
Escherichia coli Infections - microbiology
Escherichia coli Proteins - chemistry
Escherichia coli Proteins - genetics
Feces - microbiology
Fundamental and applied biological sciences. Psychology
Genetics
Humans
Infant
Isoelectric Point
Microbial Sensitivity Tests
Microbiology
Molecular Sequence Data
Sequence Analysis, DNA
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Summary:Escherichia coli CA0210 was identified in a stool culture of a 03-month-neonate in Tunisia. This strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefpirome, ceftazidime, and cefotaxime, but it remained susceptible to imipenem and cefoxitin. The beta-lactam-hydrolyzing beta-lactamase gene of E. coli CA0210 and the upstream and downstream regions were cloned, sequenced, and expressed in E. coli DH5alpha. These resistances were carried by a 1080-bp chromosomal gene that encoded a beta-lactamase with a pI of 6.3. Cloning and sequencing experiments showed that the corresponding blaTEM-15 gene was part of a chromosomally located Tn801 transposon.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2007.02.012