Reagentless Ultrasensitive Specific DNA Array Detection Based on Responsive Polymeric Biochips

Self-assembled molecular structures immobilized on solid substrates and composed of fluorophore-tagged oligonucleotide probes and an optical polymeric transducer were investigated for the trace level detection of DNA target molecules. Rapid and efficient energy transfer between the polymeric transdu...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2006-11, Vol.78 (22), p.7896-7899
Main Authors: Najari, Ahmed, Ho, Hoang Anh, Gravel, Jean-François, Nobert, Philippe, Boudreau, Denis, Leclerc, Mario
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical chemistry
Analytical, structural and metabolic biochemistry
Arrays
Base Pair Mismatch - genetics
Biological and medical sciences
Biosensing Techniques
Chemistry
Deoxyribonucleic acid
DNA
DNA - analysis
DNA - chemistry
DNA - genetics
Exact sciences and technology
Fluorescent Dyes - chemistry
Fundamental and applied biological sciences. Psychology
Molecular structure
Oligonucleotide Array Sequence Analysis - methods
Oligonucleotide Probes - analysis
Oligonucleotide Probes - genetics
Polymers
Polymorphism, Single Nucleotide - genetics
Sensitivity and Specificity
Signal transduction
Spectrometric and optical methods
Spectrometry, Fluorescence - methods
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Summary:Self-assembled molecular structures immobilized on solid substrates and composed of fluorophore-tagged oligonucleotide probes and an optical polymeric transducer were investigated for the trace level detection of DNA target molecules. Rapid and efficient energy transfer between the polymeric transducer and fluorophores within the molecular aggregates leads to a massive intrinsic amplification of the fluorescence signal and to the label-free detection of as little as 300 DNA molecules, with the specificity required for the detection of single-nucleotide mismatches. This capacity for attomolar detection levels while the sensing structures are attached onto solid supports could lead to the development of biochip platforms for fast and simple PCR-free multitarget DNA detection.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac061650+