A Single-Bead Analysis on a Disk-shaped Microfluidic Device Using an Antigen-immobilized Bead

A disk-shaped microfluidic device (lab-on-a-Disk) was developed to allow the evaluation of mental stress. As a standard sample, secretory immunoglobulin A (sIgA), which is a candidate marker of mental stress, was measured by a heterogeneous enzyme immunoassay (EIA) on the lab-on-a-Disk. Centrifugal...

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Bibliographic Details
Published in:Analytical Sciences 2007, Vol.23(8), pp.975-979
Main Authors: NAGAI, Hidenori, NARITA, Yuka, OHTAKI, Miyuki, SAITO, Keiitsu, WAKIDA, Shin-ichi
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - analysis
Antigen-Antibody Complex
Antigens - chemistry
Biomarkers - analysis
Equipment Design
Immunoglobulin A, Secretory - analysis
Immunoglobulin A, Secretory - immunology
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microspheres
Stress, Psychological - diagnosis
Stress, Psychological - immunology
Surface Properties
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Summary:A disk-shaped microfluidic device (lab-on-a-Disk) was developed to allow the evaluation of mental stress. As a standard sample, secretory immunoglobulin A (sIgA), which is a candidate marker of mental stress, was measured by a heterogeneous enzyme immunoassay (EIA) on the lab-on-a-Disk. Centrifugal force provided a microfluidic control on the lab-on-a-Disk. We examined the relationship between the rotational speed, the channel profile, and the position of the microfluidic chambers from the center of rotation to manipulate sample solutions into each reaction reservoir through microchannels sequentially, i.e., retain in a reservoir or flow into a subsequent reservoir. A single glass bead with immobilized sIgA on its surface was injected into a reservoir for a competitive antigen-antibody reaction, and applied to a specific surface in a heterogeneous assay. It is expected that the lab-on-a-Disk would be suitable for miniaturization and automation of the processes in EIA compared with a conventional EIA using a titer plate.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.23.975