Quantification of Circulating Plasma DNA Fragments as Tumor Markers in Patients with Esophageal Cancer

The quantity and quality of circulating DNA fragments was analyzed by quantitative real-time polymerase chain reactions (qPCR) in plasma from patients with esophageal carcinomas, in order to assess their diagnostic value. Patients and Methods: Plasma was collected preoperatively from 24 patients wit...

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Bibliographic Details
Published in:Anticancer research 2007-07, Vol.27 (4C), p.2737-2741
Main Authors: TOMITA, Haruhisa, ICHIKAWA, Daisuke, OTSUJI, Eigo, IKOMA, Daito, SAI, Soujin, TANI, Nobuyuki, IKOMA, Hisashi, FUJIWARA, Hitoshi, KIKUCHI, Shojiro, OKAMOTO, Kazuma, OCHIAI, Toshiya
Format: Article
Language:English
Subjects:
Aged
Biological and medical sciences
Biomarkers, Tumor - blood
Biomarkers, Tumor - genetics
Carcinoma, Squamous Cell - blood
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - pathology
Case-Control Studies
DNA, Neoplasm - blood
Esophageal Neoplasms - blood
Esophageal Neoplasms - genetics
Esophageal Neoplasms - pathology
Esophagus
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Medical sciences
Middle Aged
Neoplasm Staging
Polymerase Chain Reaction - methods
ROC Curve
Tumors
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Summary:The quantity and quality of circulating DNA fragments was analyzed by quantitative real-time polymerase chain reactions (qPCR) in plasma from patients with esophageal carcinomas, in order to assess their diagnostic value. Patients and Methods: Plasma was collected preoperatively from 24 patients with esophageal cancer and 21 healthy controls. qPCR was performed using two primer sets for the beta-actin gene, amplifying short and long segments. Results: The DNA concentrations in both the short and long assays of esophageal cancer patients were significantly higher than the controls (p
ISSN:0250-7005
1791-7530