Salmon cartilage proteoglycan modulates cytokine responses to Escherichia coli in mouse macrophages

Proteoglycans (PGs) are complex glycohydrates, which are composed of core proteins and glycosaminoglycans and widely distributed in connective tissues and on the cell surface of mammalian tissues. We investigated the effect of PG extracted from salmon cartilage on cytokine responses to stimulation w...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-12, Vol.351 (4), p.1005-1010
Main Authors: Sashinami, Hiroshi, Takagaki, Keiichi, Nakane, Akio
Format: Article
Language:English
Subjects:
Animals
Cartilage - chemistry
Cells, Cultured
Chondroitin Sulfates - pharmacology
Cytokines - metabolism
E. coli
Escherichia coli
Escherichia coli - immunology
IL-10
Interleukin-10 - metabolism
Macrophage
Macrophages - drug effects
Macrophages - immunology
Macrophages - microbiology
Mice
Phosphorylation
Proteoglycan
Proteoglycans - isolation & purification
Proteoglycans - pharmacology
Salmon - anatomy & histology
Salmon - metabolism
Salmonidae
STAT3 Transcription Factor - metabolism
TNF-α
Toll-Like Receptor 4 - antagonists & inhibitors
Toll-Like Receptor 4 - metabolism
Tumor Necrosis Factor-alpha - metabolism
Up-Regulation
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Summary:Proteoglycans (PGs) are complex glycohydrates, which are composed of core proteins and glycosaminoglycans and widely distributed in connective tissues and on the cell surface of mammalian tissues. We investigated the effect of PG extracted from salmon cartilage on cytokine responses to stimulation with heat-killed Escherichia coli (HKEC) in a mouse macrophage cell line, RAW264.7. PG exhibited the suppression of tumor necrosis factor-α production compared with chondroitin 4 sulfate (C4S) and chondroitin 6 sulfate (C6S). PG also revealed the up-regulation of interleukin-10 production. HKEC-induced Toll-like receptor 4 (TLR4) and inducible nitric oxide synthase expression was dose-dependently suppressed by treatment with PG, C4S or C6S, and the PG showed the strongest suppressive effect among 3 compounds. Only PG dramatically up-regulated the expression of signal transducer and activator of transcription 3 (STAT3), and the phosphorylation of STAT3 in mouse macrophages. Our results suggested that the novel interaction might exist between the extracellular matrix and immune system.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.10.146