Two new lipid-dependent Malassezia species from domestic animals

• Published in: FEMS yeast research 2007-09, Vol.7 (6), p.1064-1076
• Main Authors: Cabañes, F. Javier, Theelen, Bart, Castellá, Gemma, Boekhout, Teun
• Format: Article
• Language: English
• Subjects: Amplified fragment length polymorphism; Animals; Animals, Domestic - microbiology; asexual speciation; Chitin; Chitin synthase; DNA, Ribosomal Spacer - genetics; DNA-directed RNA polymerase; Domestic animals; Goats; Horses; Lipid Metabolism; Malassezia; Malassezia - classification; Malassezia - cytology; Malassezia - genetics; Malassezia - isolation & purification; Malassezia caprae; Malassezia equina; New species; New taxa; Phylogeny; Physical characteristics; RNA polymerase; RNA, Ribosomal - genetics; rRNA; rRNA 26S; rRNA 5.8S; skin; Skin - microbiology; Species Specificity; taxonomy
• ISSN: 1567-1356; 1567-1364
• DOI: 10.1111/j.1567-1364.2007.00217.x

Abstract During a study on the occurrence of lipid-dependent Malassezia spp. in domestic animals, some atypical strains, phylogenetically related to Malassezia sympodialis Simmons et Guého, were shown to represent novel species. In this study, we describe two new taxa, Malassezia caprae sp. nov. (type strain MA383=CBS 10434), isolated mainly from goats, and Malassezia equina sp. nov. (type strain MA146=CBS 9969), isolated mainly from horses, including their morphological and physiological characteristics. The validation of these new taxa is further supported by analysis of the D1/D2 regions of the 26S rRNA gene, the ITS1–5.8S–ITS2 rRNA, the RNA polymerase subunit 1 and chitin synthase nucleotide sequences, and the amplified fragment length polymorphism patterns, which were all consistent in separating these new species from the other species of the genus, and those of the M. sympodialis species cluster, specifically.