Rapid Determination of Hydrogen Peroxide Using Peroxidase Immobilized on Amberlite IRA-743 and Minerals in Honey

• Published in: Journal of agricultural and food chemistry 2007-08, Vol.55 (17), p.6885-6890
• Main Authors: de Abreu Franchini, Rômulo Augusto, de Souza, Cabrini Ferraz, Colombara, Rosana, Costa Matos, Maria Auxiliadora, Matos, Renato Camargo
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; calcium; capillary zone electrophoresis; Confectionery products and chocolate industries, honey; enzyme activity; Enzymes, Immobilized; food analysis; Food industries; Fundamental and applied biological sciences. Psychology; honey; Honey - analysis; hydrogen peroxide; Hydrogen Peroxide - analysis; immobilized enzymes; lithium; magnesium; manganese; oxidation; Peroxidase; potassium; resins; Resins, Synthetic; sodium; Trace Elements - analysis
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf071062c

Hydrogen peroxide and trace metals (K+, Ca2+, Na+, Mg2+, Mn2+, and Li+) were determined in 14 samples of Brazilian commercial honeys. The method for the determination of H2O2 is based on selective oxidation of H2O2 using an on-line tubular reactor containing peroxidase immobilized on Amberlite IRA-743 resin. Reactors presented high stability for at least 2 weeks under intense use. The results show a simple, accurate, selective, and readily applied method to the determination of H2O2 in honey. The trace metals were determined by capillary zone eletrophoresis. Mean contents of 656, 69.1, 71.8, 36.0, 21.4, and 1.70 mg/kg were found, respectively, for K+, Ca2+, Na+, Mg2+, Mn2+, and Li+ in the analyzed honeys. The cations were identified by comparison of the relative migration times of their peaks with the Ba2+ migration time used as reference. The electrophoretic analysis was simple and rapid and did not require any other preparation of sample than dilution and filtration. Keywords: Capillary zone eletrophoresis; honey; hydrogen peroxide; peroxidase immobilized; trace metals