One-step Nucleic Acid Amplification for Intraoperative Detection of Lymph Node Metastasis in Breast Cancer Patients

• Published in: Clinical cancer research 2007-08, Vol.13 (16), p.4807-4816
• Main Authors: TSUJIMOTO, Masahiko, NAKABAYASHI, Kadzuki, TAMAKI, Yasuhiro, NOGUCHI, Shinzaburo, KATAOKA, Tatsuki R, NAKAJIMA, Hiromu, KOMOIKE, Yoshifumi, INAJI, Hideo, TSUGAWA, Koichiro, SUZUKI, Koyu, NAKAMURA, Seigo, DAITOH, Motonari, YOSHIDOME, Katsuhide, OTOMO, Yasuhiro, MATSUURA, Nariaki, KANEKO, Tomoyo, IWASE, Takuji, AKIYAMA, Futoshi, KATO, Yo, TSUDA, Hitoshi, UEDA, Shigeto, SATO, Kazuhiko
• Format: Article
• Language: English
• Subjects: Antineoplastic agents; Base Sequence; Biological and medical sciences; Breast cancer; Breast Neoplasms - chemistry; Breast Neoplasms - pathology; Breast Neoplasms - surgery; Female; Gynecology. Andrology. Obstetrics; Hematologic and hematopoietic diseases; Humans; Immunohistochemistry; Intraoperative Period; Keratin-19 - analysis; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymph node; Lymphatic Metastasis; Mammary gland diseases; Medical sciences; Metastasis; Molecular Sequence Data; Nucleic acid amplification; Nucleic Acid Amplification Techniques - methods; OSNA; Pharmacology. Drug treatments; Tumors
• ISSN: 1078-0432; 1557-3265
• DOI: 10.1158/1078-0432.CCR-06-2512

Purpose: Detection of sentinel lymph node (SLN) metastasis in breast cancer patients has conventionally been determined by intraoperative histopathologic examination of frozen sections followed by definitive postoperative examination of permanent sections. The purpose of this study is to develop a more efficient method for intraoperative detection of lymph node metastasis. Experimental Design: Cutoff values to distinguish macrometastasis, micrometastasis, and nonmetastasis were determined by measuring cytokeratin 19 (CK19) mRNA in histopathologically positive and negative lymph nodes using one-step nucleic acid amplification (OSNA). In an intraoperative clinical study involving six facilities, 325 lymph nodes (101 patients), including 81 SLNs, were divided into four blocks. Alternate blocks were used for the OSNA assay with CK19 mRNA, and the remaining blocks were used for H&E and CK19 immunohistochemistry–based three-level histopathologic examination. The results from the two methods were then compared. Results: We established CK19 mRNA cutoff values of 2.5 × 10 2 and 5 × 10 3 copies/μL. In the clinical study, an overall concordance rate between the OSNA assay and the three-level histopathology was 98.2%. Similar results were obtained with 81 SLNs. The OSNA assay discriminated macrometastasis from micrometastasis. No false positive was observed in the OSNA assay of 144 histopathologically negative lymph nodes from pN0 patients, indicating an extremely low false positive for the OSNA assay. Conclusion: The OSNA assay of half of a lymph node provided results similar to those of three-level histopathology. Clinical results indicate that the OSNA assay provides a useful intraoperative detection method of lymph node metastasis in breast cancer patients.