Donor-Reactive Cytokine Production After HLA-Identical Living Related Kidney Transplantation: A Protein-Array Analysis

In the present pilot study, we investigated which proteins are produced after donor stimulation of peripheral blood mononuclear cells from recipients of HLA-identical living related kidney transplant. We used a protein-array analysis to determine cytokines, chemokines, and growth factors in supernat...

Full description

Saved in:
Bibliographic Details
Published in:Transplantation proceedings 2006-11, Vol.38 (9), p.2825-2827
Main Authors: Gerrits, J.H., van de Wetering, J., Weimar, W., van Besouw, N.M.
Format: Article
Language:English
Subjects:
Chemokines - genetics
Cytokines - genetics
Family
Glomerulonephritis, Membranous - immunology
Glomerulonephritis, Membranous - surgery
Growth Substances - genetics
Histocompatibility Testing
Humans
Interleukins - genetics
Kidney Transplantation - immunology
Kidney Transplantation - physiology
Living Donors
Pilot Projects
Protein Array Analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present pilot study, we investigated which proteins are produced after donor stimulation of peripheral blood mononuclear cells from recipients of HLA-identical living related kidney transplant. We used a protein-array analysis to determine cytokines, chemokines, and growth factors in supernatant from donor-stimulated mixed lymphocyte reaction cultures. Autologous cultures were considered to be negative controls. In 38 out of 42 proteins (90%), the donor response was higher compared with the autologous response. Therefore, we concluded that even after HLA-identical living related kidney transplantation we could measure a donor-reactive response, which we assumed was directed toward minor histocompatibility or non-HLA antigens.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2006.08.183