Simultaneous characterization of isoflavonoids and astragalosides in two Astragalus species by high-performance liquid chromatography coupled with atmospheric pressure chemical ionization tandem mass spectrometry

A method was developed for the simultaneous identification of astragalosides (AGs) and isoflavonoids (IFs) in the roots of Astragalus membranaceus and Astragalus mongholicus by HPLC coupled with atmospheric pressure chemical ionization MS/MS (HPLC‐APCI‐MS/MS). Diagnostic fragment ions of AGs and dif...

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Bibliographic Details
Published in:Journal of separation science 2007-08, Vol.30 (13), p.2059-2069
Main Authors: Zhang, Xi, Xiao, Hong-Bin, Xue, Xing-Ya, Sun, Yan-Guo, Liang, Xin-Miao
Format: Article
Language:English
Subjects:
Astragaloside
Astragalus membranaceus
Astragalus mongholicus
Astragalus Plant - chemistry
Atmospheric Pressure
Biological and medical sciences
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
General pharmacology
HPLC-APCI/MS/MS
Isoflavone
Isoflavones - analysis
Isoflavones - isolation & purification
Medical sciences
Molecular Structure
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - chemistry
Saponins - analysis
Saponins - isolation & purification
Tandem Mass Spectrometry - instrumentation
Tandem Mass Spectrometry - methods
Triterpenes - analysis
Triterpenes - isolation & purification
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Summary:A method was developed for the simultaneous identification of astragalosides (AGs) and isoflavonoids (IFs) in the roots of Astragalus membranaceus and Astragalus mongholicus by HPLC coupled with atmospheric pressure chemical ionization MS/MS (HPLC‐APCI‐MS/MS). Diagnostic fragment ions of AGs and different group of IFs were obtained with one AG and eight IF standards analyzed by CID‐MS, which were adopted as characteristic MS/MS fingerprints for further identification of these compounds in the two Astragalus species by using HPLC‐APCI‐MS/MS. A total of 20 IFs and 10 AGs were identified or tentatively identified. Among them, six IFs were detected in A. membranaceus for the first time and five IFs were firstly identified in A. mongholicus. The results indicate that HPLC‐APCI‐MS/MS is a powerful tool for the simultaneous characterization of IFs and AGs in complex matrix.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200700014