Soluble Starch Synthase I Effects Differences in Amylopectin Structure between indica and japonica Rice Varieties

The effect of soluble starch synthase I (SSI) on differences of amylopectin structure between the indica and japonica rice varieties was investigated. Native-PAGE/active staining analysis showed that the SSI activity of an indica rice variety, ‘Kasalath', was significantly lower than that of a...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2006-11, Vol.54 (24), p.9234-9240
Main Authors: Takemoto-Kuno, Yoko, Suzuki, Keitaro, Nakamura, Sumiko, Satoh, Hikaru, Ohtsubo, Keníchi
Format: Article
Language:English
Subjects:
amylopectin
Amylopectin - biosynthesis
Amylopectin - chemistry
Biological and medical sciences
Blotting, Northern
carbohydrate structure
cell wall components
Cereal and baking product industries
chemical constituents of plants
corn
DNA, Complementary - chemistry
Electrophoresis, Polyacrylamide Gel
enzyme activity
Food industries
Fundamental and applied biological sciences. Psychology
gene expression
Humans
inbred lines
insect growth
insect pests
larvae
leaf sheaths
leaves
messenger RNA
Oryza - genetics
Oryza - metabolism
Oryza sativa
pest resistance
phenylpropanoids
Polymorphism, Restriction Fragment Length
rice
Sesamia nonagrioides
soluble starch synthase I
starch synthase
Starch Synthase - metabolism
varieties
Zea mays
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Summary:The effect of soluble starch synthase I (SSI) on differences of amylopectin structure between the indica and japonica rice varieties was investigated. Native-PAGE/active staining analysis showed that the SSI activity of an indica rice variety, ‘Kasalath', was significantly lower than that of a japonica rice variety, ‘Nipponbare', and that the low activity in ‘Kasalath' was maintained during seed development. The result of northern blot analyses suggests that the low expression of SSI in ‘Kasalath' is controlled at the transcription levels of SSI mRNA. Chain length distribution of amylopectin in F3 endosperms derived from a cross between two varieties showed that not only SSIIa but also SSI regulated the population of short chains. These results indicate that the low activity of SSI gives rise to the decrease of short chains in amylopectin of indica rice varieties, suggesting that SSI effects the differences in physicochemical properties between two varieties. Keywords: Soluble starch synthase I; indica rice variety; japonica rice variety; amylopectin
ISSN:0021-8561
1520-5118
DOI:10.1021/jf061200i