Urine sediment examination: A comparison of automated urinalysis systems and manual microscopy

Several automated instruments examining urine sediment have been introduced. We compared the performance of Sysmex UF-100 and Iris iQ200 with manual microscopy in urine sediment testing. Four hundred and thirty-six urine samples were collected. The urine sediments were examined by manual microscopy...

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Bibliographic Details
Published in:Clinica chimica acta 2007-09, Vol.384 (1-2), p.28-34
Main Authors: Chien, Tzu-I, Kao, Jau-Tsuen, Liu, Hui-Lan, Lin, Po-Chang, Hong, Jhih-Sian, Hsieh, Han-Peng, Chien, Miao-Ju
Format: Article
Language:English
Subjects:
Autoanalysis - instrumentation
Automation
Humans
Microscopy
Urinalysis - instrumentation
Urine - chemistry
Urine microscopy
Urine sediment
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Summary:Several automated instruments examining urine sediment have been introduced. We compared the performance of Sysmex UF-100 and Iris iQ200 with manual microscopy in urine sediment testing. Four hundred and thirty-six urine samples were collected. The urine sediments were examined by manual microscopy and these 2 automated urinalysis systems. The within-run CVs for urine samples ranged from 3.4% to 22.3% for the iQ200, 1.6% to 24.2% for the UF-100 and 12.5% to 43.9% for manual microscopy. Between-run CVs on quality-control samples ranged from 6.1% to 32.4% for the iQ200 and 3.5% to 24.7% for the UF-100. The agreement between methods was good for red blood cells and white blood cells counts based on r values of 0.935 to 0.968. However, for epithelial cells, the values measured by different systems were poorly correlated (r=0.888–0.922). The Bland–Altman plot indicated a trend towards the automated cell count being greater than the manual microscopy as the epithelial cell count increased. Casts were difficultly differentiated by 2 automated systems. These 2 automated urinalysis systems demonstrated good concordance with each other in urine sediment examination. The automated process could be used as a screening procedure but some manual microscopy was still necessary.
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2007.05.012