Advances in nucleic acid-based diagnostics of bacterial infections

Methods for rapid detection of infectious bacteria and antimicrobial-resistant pathogens have evolved significantly over the last decade. Many of the new procedures are nucleic acid-based and replace conventional diagnostic methods like culturing which is time consuming especially with fastidious an...

Full description

Saved in:
Bibliographic Details
Published in:Clinica chimica acta 2007-09, Vol.384 (1-2), p.1-11
Main Authors: Barken, Kim B., Haagensen, Janus A.J., Tolker-Nielsen, Tim
Format: Article
Language:English
Subjects:
Bacterial Infections - diagnosis
DNA
DNA, Bacterial - analysis
FISH
Humans
In Situ Hybridization
Microarray
Molecular diagnostic
Oligonucleotide Array Sequence Analysis
PCR
Polymerase Chain Reaction - methods
RNA, Bacterial - analysis
RNA, Ribosomal, 16S - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Methods for rapid detection of infectious bacteria and antimicrobial-resistant pathogens have evolved significantly over the last decade. Many of the new procedures are nucleic acid-based and replace conventional diagnostic methods like culturing which is time consuming especially with fastidious and slow growing microorgansims. The widespread use of antibiotics has resulted in an increased number of cases with resistant microorganisms such as methicillin-resistant Staphylococcus aureus, vancomycin resistant enterococci, and multidrug-resistant Mycobacterium tuberculosis. Rapid detection of these pathogens is important to isolate patients and prevent further spreading of the diseases. Newly developed diagnostic procedures are superior with respect to turnaround time, sensitivity and specificity. Methods like multiplex real time PCR and different array-based technologies offer the possibility of multiparameter assays where several pathogens and antibiotic resistance genes can be detected simultaneously.
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2007.07.004