High expression of transgene protein in Spirodela

The monocot family Lemnaceae (duckweed) is composed of small, edible, aquatic plants. Spirodela oligorrhiza SP is a duckweed with a biomass doubling time of about 2 days under controlled, axenic conditions. Stably transformed Spirodela plants were obtained following co-cultivation of regenerative ca...

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Bibliographic Details
Published in:Plant cell reports 2007-09, Vol.26 (9), p.1511-1519
Main Authors: VUNSH, Ron, JIHONG LI, HANANIA, Uri, EDELMAN, Marvin, FLAISHMAN, Moshe, PERL, Avihai, WISNIEWSKI, Jean-Pierre, FREYSSINET, Georges
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
Antibiotic resistance
Aquatic plants
Araceae - metabolism
Biological and medical sciences
Biotechnology
Blotting, Southern
Deoxyribonucleic acid
DNA
Floating plants
Fundamental and applied biological sciences. Psychology
Gene expression
Genetic engineering
Genetic technics
Genome, Plant
Kanamycin Resistance
Lemnaceae
Methods. Procedures. Technologies
Microscopy, Fluorescence
Mutation - genetics
Plant Proteins - metabolism
Plasmids
Spirodela
Spirodela oligorrhiza
Subcellular Fractions - metabolism
Transformation, Genetic
Transgenes
Transgenic animals and transgenic plants
Transgenic plants
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Summary:The monocot family Lemnaceae (duckweed) is composed of small, edible, aquatic plants. Spirodela oligorrhiza SP is a duckweed with a biomass doubling time of about 2 days under controlled, axenic conditions. Stably transformed Spirodela plants were obtained following co-cultivation of regenerative calli with Agrobacterium tumefaciens. GFP activity was successfully monitored in different subcellular compartments of the plant and correlated with different targeting sequences. Transgenic lines were followed for a period of at least 18 months and more than 180 vegetative doublings (generations). The lines are stable in morphology, growth rate, transgene expression, and activity as measured by DNA-DNA and immunoblot hybridizations, fluorescence activity measurements, and antibiotic resistance. The level of transgene expression is a function of leader sequences rather than transgene copy number. A stable, transgenic, GFP expression level >25% of total soluble protein is demonstrated for the S. oligorrhiza system, making it among the higher expressing systems for nuclear transformation in a higher plant.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-007-0361-4