Gas chromatographic–mass spectrometric analysis of d-limonene in human plasma

d-Limonene shows carminative and cholagogue effects and is used in treatment of gallstone, cholecystitis and angiocholitis. A simple method was developed to determine the concentration of d-limonene in human plasma. d-Limonene and internal standard (naphthalane, C 10H 18) were extracted with n-hexan...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2007-09, Vol.44 (5), p.1095-1099
Main Authors: Wang, Shurong, Chen, Yu, Gao, Zhaoxia, Xiong, Meijin, Zhong, Zhirong, Ye, Liming
Format: Article
Language:English
Subjects:
Adolescent
Adult
Analysis
Analytical, structural and metabolic biochemistry
Area Under Curve
Biological and medical sciences
Calibration
Cyclohexenes - blood
Cyclohexenes - chemistry
Cyclohexenes - pharmacokinetics
d-Limonene
Drug Stability
Freezing
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry - instrumentation
Gas Chromatography-Mass Spectrometry - methods
GC–MS
General pharmacology
Half-Life
Human plasma
Humans
Male
Medical sciences
Metabolic Clearance Rate
Molecular Structure
Pharmacology. Drug treatments
Reference Standards
Sensitivity and Specificity
Temperature
Terpenes - blood
Terpenes - chemistry
Terpenes - pharmacokinetics
Time Factors
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Summary:d-Limonene shows carminative and cholagogue effects and is used in treatment of gallstone, cholecystitis and angiocholitis. A simple method was developed to determine the concentration of d-limonene in human plasma. d-Limonene and internal standard (naphthalane, C 10H 18) were extracted with n-hexane and then injected to GC–MS. Calibration curves were linear ( r = 0.9990, n = 6) in the range of 2–500 ng/ml for d-limonene in human plasma. Limit of detection and quantification were 0.5 and 2 ng/ml, respectively. This rapid and specific method was applied to the clinical pharmacokinetic investigation of d-limonene.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2007.04.018