Chloroform extract of deer antler inhibits osteoclast differentiation and bone resorption

• Published in: Journal of ethnopharmacology 2007-09, Vol.113 (2), p.191-198
• Main Authors: Li, Yin-Ji, Kim, Tae-Hee, Kwak, Han Bok, Lee, Zang Hee, Lee, Soo-Young, Jhon, Gil-Ja
• Format: Article
• Language: English
• Subjects: Actins - metabolism; Adaptor Proteins, Signal Transducing - metabolism; animal tissue extracts; Animals; antlers; Antlers - chemistry; Apoptosis - drug effects; bioassays; Biological and medical sciences; bone marrow; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Bone resorption; Bone Resorption - metabolism; Bone Resorption - pathology; Bone Resorption - prevention & control; cell differentiation; Cell Differentiation - drug effects; Cell Survival - drug effects; Cells, Cultured; Cervidae; Cervus elaphus; chemical composition; Chloroform - chemistry; Deer; Dose-Response Relationship, Drug; Extracellular Signal-Regulated MAP Kinases - metabolism; General pharmacology; Macrophages - cytology; Macrophages - drug effects; Macrophages - metabolism; Medical sciences; medicinal properties; Mice; Mice, Inbred C57BL; NF-kappa B - metabolism; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteoclast; osteoclasts; Osteoclasts - cytology; Osteoclasts - drug effects; Osteoclasts - metabolism; Osteoporosis; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phosphorylation - drug effects; Proto-Oncogene Proteins c-akt - metabolism; RANK Ligand - pharmacology; Tissue Extracts - chemistry; Tissue Extracts - isolation & purification; Tissue Extracts - pharmacology
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2007.04.020

It has been reported that deer antler extract has anti-bone resorptive activity in vivo. However, little is known about the cellular and molecular mechanism of this effect. In this study, we investigated the effects of deer antler extracts on osteoclast differentiation and bone-resorption in vitro. Chloroform extract (CE-C) of deer antler inhibited osteoclast differentiation in mouse bone marrow cultures stimulated by receptor activator of NF-κB ligand (RANKL) and macrophage-colony stimulating factor (M-CSF). CE-C suppressed the activation of extracellular signal-regulated kinase (ERK), protein kinase B (PKB/Akt) and inhibitor of kappa B (I-κB) by RANKL in osteoclast precursor cells. It also inhibited the bone resorptive activity of differentiated osteoclasts that was accompanied by disruption of actin rings and induction of the apoptosis. These results demonstrate deer antler extract may be a useful remedy for the treatment of bone-resorption diseases such as osteoporosis.