FcR gamma-chain dependent signaling in immature neutrophils is mediated by FcalphaRI, but not by FcgammaRI

Neutrophil-mediated tumor cell lysis is more efficiently triggered by FcalphaRI (CD89), than by FcgammaRI (CD64). This difference is most evident in immature neutrophils in which FcgammaRI-mediated tumor cell lysis is absent. In this study, we show that FcR gamma-chain-dependent functions (such as A...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of immunology (1950) 2007-09, Vol.179 (5), p.2918-2924
Main Authors: Otten, Marielle A, Leusen, Jeanette H W, Rudolph, Esther, van der Linden, Joke A, Beelen, Robert H J, van de Winkel, Jan G J, van Egmond, Marjolein
Format: Article
Language:English
Subjects:
Animals
Antigens, CD - metabolism
Bone Marrow - immunology
Cell Line, Tumor
Humans
Immunoprecipitation
Mice
Neutrophils - immunology
Receptors, Fc - metabolism
Receptors, IgG - metabolism
Respiratory Burst
Signal Transduction
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Neutrophil-mediated tumor cell lysis is more efficiently triggered by FcalphaRI (CD89), than by FcgammaRI (CD64). This difference is most evident in immature neutrophils in which FcgammaRI-mediated tumor cell lysis is absent. In this study, we show that FcR gamma-chain-dependent functions (such as Ab-dependent cellular cytotoxicity and respiratory burst), as well as signaling (calcium mobilization and MAPK phosphorylation), were potently triggered via FcalphaRI, but not via FcgammaRI, in immature neutrophils. Internalization, an FcR gamma-chain-independent function, was, however, effectively initiated via both receptors. These data suggest an impaired functional association between FcgammaRI and the FcR gamma-chain, which prompted us to perform coimmunoprecipitation experiments. As a weaker association was observed between FcgammaRI and FcR gamma-chain, compared with FcalphaRI and FcR gamma-chain, our data support that differences between FcalphaRI- and FcgammaRI-mediated functions are attributable to dissimilarities in association with the FcR gamma-chain.
ISSN:0022-1767