Analysis of ghost cells in calcifying cystic odontogenic tumors by confocal laser scanning microscopy

Objective The confocal laser scanning microscope represents an effective tool for studying biological samples stained for fluorescence observation. In this study we have used the confocal microscope to analyze ghost cells in calcifying cystic odontogenic tumors. Study design Specimens from 15 calcif...

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Published in:Oral surgery, oral medicine, oral pathology, oral radiology and endodontics oral medicine, oral pathology, oral radiology and endodontics, 2007-09, Vol.104 (3), p.391-394
Main Authors: Lucchese, Alberta, DMD, PhD, Scivetti, Michele, DMD, Pilolli, Giovanni Pietro, DMD, Favia, Gianfranco, MD, PhD
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Biological and medical sciences
Child
Child, Preschool
Dentistry
Female
Fluorescence
Humans
Jaw Neoplasms - diagnostic imaging
Jaw Neoplasms - pathology
Jaw Neoplasms - ultrastructure
Male
Medical sciences
Microscopy, Confocal
Middle Aged
Odontogenic Cyst, Calcifying - diagnostic imaging
Odontogenic Cyst, Calcifying - pathology
Odontogenic Cyst, Calcifying - ultrastructure
Otorhinolaryngology. Stomatology
Radiography
Surgery
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Summary:Objective The confocal laser scanning microscope represents an effective tool for studying biological samples stained for fluorescence observation. In this study we have used the confocal microscope to analyze ghost cells in calcifying cystic odontogenic tumors. Study design Specimens from 15 calcifying cystic odontogenic tumor cases were stained with hematoxylin and eosin, and scanned by a confocal laser scanning microscope to generate optically sectioned images. Results All of the analyzed samples presented autofluorescent cells that were identified as ghost cells. The degree of autofluorescence intensity was variable and may be a result of the presence of hard keratin. Conclusion The confocal laser scanning microscope may be of help in analyzing and defining the nature and extent of keratinization processes in calcifying cystic odontogenic tumor ghost cells.
ISSN:1079-2104
1528-395X
DOI:10.1016/j.tripleo.2006.09.005