Characterization of PAF-AH Ib1 in NOD mice: PAF-AH may not be a candidate gene of the diabetes susceptibility Idd4.1 locus

• Published in: Annals of the New York Academy of Sciences 2006-10, Vol.1079 (1), p.147-152
• Main Authors: Mi, Qing-Sheng, Zhou, Li, Grattan, Marsha, Wang, Zai-Zhao, Sivilotti, M, She, Jing-Xiang, Delovitch, Terry L
• Format: Article
• Language: English
• Subjects: 1-Alkyl-2-acetylglycerophosphocholine Esterase - genetics; Animals; Chromosome Mapping; Diabetes Mellitus, Type 1 - genetics; Diabetes Mellitus, Type 1 - immunology; Diabetes Mellitus, Type 1 - physiopathology; Genetic Markers; Genetic Predisposition to Disease; Mice; Mice, Inbred NOD; Mice, Mutant Strains; Mice, SCID
• ISSN: 0077-8923; 1749-6632
• DOI: 10.1196/annals.1375.022

We recently mapped Idd4 to a 5.2 cM interval on chromosome 11 with two subloci, Idd4.1 and Idd4.2, in nonobese diabetic (NOD) mice. Based on the localization of platelet-activating factor acetylhydrolase Ib1 (PAF-AHIb1) and the decreased activity of PAF-AH in type 1 diabetes (T1D) patients, we hypothesized that PAF-AHIb1 in Idd4.1 is a candidate gene. The PAF-AHIb1 gene in NOD mice was cloned and sequenced, and its expression and function were studied. No polymorphisms were detected in PAF-AHIb1 cDNA between NOD and B6 mice. The expression of PAF-AH Ib1 at the mRNA and protein levels was found to be similar in different tissues between NOD and B6 mice. PAF-AH activity does not differ in the pancreatic islets or spleen between NOD and B6 mice. Our findings suggest that PAF-AH Ib1 may not be a diabetes-susceptibility gene in the Idd4.1 sublocus.