nDEP microwells for single-cell patterning in physiological media

We present a novel technique to accurately position single cells on a substrate using negative dielectrophoresis and cell-substrate adhesion. The cells are suspended in physiological media throughout the patterning process. We also verify the biocompatibility of this method by demonstrating that the...

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Bibliographic Details
Published in:Lab on a chip 2007-01, Vol.7 (9), p.1146-1153
Main Authors: Mittal, Nikhil, Rosenthal, Adam, Voldman, Joel
Format: Article
Language:English
Subjects:
Cell Separation - instrumentation
Cell Separation - methods
Cells, Cultured
Electrophoresis - instrumentation
Electrophoresis - methods
Equipment Design
Equipment Failure Analysis
Flow Cytometry - instrumentation
Flow Cytometry - methods
HL-60 Cells
Humans
Microelectrodes
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Stem Cells - cytology
Stem Cells - physiology
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Summary:We present a novel technique to accurately position single cells on a substrate using negative dielectrophoresis and cell-substrate adhesion. The cells are suspended in physiological media throughout the patterning process. We also verify the biocompatibility of this method by demonstrating that the patterned cells proliferate and show normal morphology. We calculate the temperatures and transmembrane potential that cells in the device experience and compare them to physiologically acceptable levels described in previous studies.
ISSN:1473-0197
1473-0189
DOI:10.1039/b706342c