Expression of Pituitary Gland Genes in Laying Geese

The purpose of this study was to detect differential expression of genes in the pituitary gland in laying geese by suppression subtractive hybridization (SSH). Pituitary glands from prelaying and laying geese were dissected for mRNA extraction. The cDNA from pituitary glands of prelaying geese was s...

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Bibliographic Details
Published in:Poultry science 2006-12, Vol.85 (12), p.2265-2269
Main Authors: Yen, C.F, Lin, H.W, Hsu, J.C, Lin, C, Shen, T.F, Ding, S.T
Format: Article
Language:English
Subjects:
animal age
Animals
complementary DNA
Female
females
geese
Geese - genetics
Geese - physiology
gene expression
Gene Expression Profiling - veterinary
Gene Expression Regulation
genomics
messenger RNA
molecular sequence data
nucleotide sequences
oviposition
Oviposition - physiology
pituitary gland
Pituitary Gland - metabolism
prolactin
sequence analysis
suppression subtractive hybridization
visinin
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Summary:The purpose of this study was to detect differential expression of genes in the pituitary gland in laying geese by suppression subtractive hybridization (SSH). Pituitary glands from prelaying and laying geese were dissected for mRNA extraction. The cDNA from pituitary glands of prelaying geese was subtracted from the cDNA from the pituitary glands of laying geese (forward subtraction); the reverse subtraction was also performed. We screened 384 clones with possible differentially expressed gene fragments by differential screening. Sixty-five clones from the differential screening results were subjected to gene sequencing and further analysis. We found that at least 19 genes were highly expressed in the pituitary glands of laying geese compared with prelaying geese. Among these, 6 genes (including 4 novel genes) were confirmed by virtual Northern analysis. We found that prolactin and visinin-like protein were highly expressed in the pituitary glands of laying geese compared with prelaying geese (P < 0.05). Further investigation is needed to demonstrate specific functions of the novel genes discovered in the current study.
ISSN:0032-5791
1525-3171
DOI:10.1093/ps/85.12.2265